Lipid Assay Kit

Cosmo Bio

SKU: PMC-AK09F

Detection of Adipocyte Differentiation Adipocytes synthesize neutral fat and accumulate it as fat globules in the cell during the development from precursor cells to mature adipocytes. The fat globules can be a indicator of the differentiation status of adipocytes.

This kit is a staining kit for fat globules in the adipocyte by oil red 0. Moreover, red-stained fat globules can be isolated and measured with determination of pigment.

Citations for Lipid Assay Kit – 5 Found

Watanabe, Yasuharu; Nagai, Yoshinori; Honda, Hiroe; Okamoto, Naoki; Yamamoto, Seiji; Hamashima, Takeru; Ishii, Yoko; Tanaka, Miyako; Suganami, Takayoshi; Sasahara, Masakiyo; Miyake, Kensuke; Takatsu, Kiyoshi. Isoliquiritigenin Attenuates Adipose Tissue Inflammation in vitro and Adipose Tissue Fibrosis through Inhibition of Innate Immune Responses in Mice. Scientific Reports. 2016;6( 26975571):23097. 

Isono, Takahiro; Chano, Tokuhiro; Yonese, Junji; Yuasa, Takeshi. Therapeutic inhibition of mitochondrial function induces cell death in starvation-resistant renal cell carcinomas. Scientific Reports. 2016;6( 27157976):25669. 

Kawano, Shodai; Maruyama, Junichi; Nagashima, Shunta; Inami, Kazutoshi; Qiu, Wenzhe; Iwasa, Hiroaki; Nakagawa, Kentaro; Ishigami-Yuasa, Mari; Kagechika, Hiroyuki; Nishina, Hiroshi; Hata, Yutaka. A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. Journal Of Biochemistry. 2015;158(5):413-23. 
- Comments: Cosmo Bio’s Lipid Assay Kit was used in a study that validated the most suitable reference genes for 3T3-L1 adipocyte qRT-PCR studies under both differentiation and non-differentiation conditions.

Cahyadi, Danang Dwi; Warita, Katsuhiko; Takeda-Okuda, Naoko; Tamura, Jun-Ichi; Hosaka, Yoshinao Z. Qualitative and quantitative analyses in sulfated glycosaminoglycans, chondroitin sulfate/dermatan sulfate, during 3 T3-L1 adipocytes differentiation. Animal Science Journal = Nihon Chikusan Gakkaiho. 2023;94(1):e13894. 
- Comments: Cosmo Bio’s Lipid Assay Kit was used in a study that used high-performance liquid chromatography and real-time PCR to analyze the chondroitin sulfate/dermatan sulfate profile as well as gene expression associated with their synthesis and degradation in 3T3-L1 cells before and after adipogenic induction.

Cahyadi, Danang Dwi; Warita, Tomoko; Irie, Nanami; Mizoguchi, Kana; Tashiro, Jiro; Hosaka, Yoshinao Z; Warita, Katsuhiko. Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells. Adipocyte. 2023;12(1):2235081.