KBM EV Pure

Kohjin Bio

Catalog No.: KJN-16050700

KBM EV Pure (Serum-free medium for Exosome production)

This Product is a medium for producing exosome derived from mesenchymal stem cell. After mesenchymal stem cells are grown in cell culture medium, they can be replaced
with KBM EV Pure to efficiently produce exosomes. Any type of cell growth medium is acceptable.

Features

• Animal Origin Free(AOF)and Serum Free culture medium.
  Lower the risk of infectious diseases caused by materials derived from different animal species.
• Low-protein Composition
  As purifying exosomes, contamination of proteins derived from the culture medium can be suppressed.
• Phenol red free
• Chemically Defined medium

Applicable cell source of exosome

• Canine Adipose-derived MSCs
• Canine Bone marrow-derived MSCs
• Human Adipose-derived MSCs
• Human Bone marrow-derived MSCs etc.

Example of use

• Seed cells
  72 h　Culture cells in cell culture medium (serum or serum-free medium)
• Replace Medium to KBM EV Pure
  24-48 h　Maintain cells and produce exosome
• Collect culture supernatant solution
• Purify exosomes

Data

Immediately after medium replacement	After 48 hours of maintenance

Fig.1 Morphology of cells maintained in KBM EV Pure 

Purified 9mL of supernatant fluid sample
Lane(A) PC(Human-derived)
Lane(B) Culture supernatant (before purification)
Lane2 to 9 Elution fraction#2 to 9 1.0mL
Lane1 and 10 Elution fraction#1 and #10 0.5mL

Sample 2-10 mixed and concentrated (100 μL collection)
Lane(C) Before Concentration （Elution fractions 2-10 mix）
Lane(D) After Concentration （Elution fractions 2-10 mix）
Fig. 2：Surface marker expression


Fig. 3：Particle concentration and size distribution of EVs

Data Provided by：Prof. Kazuya Edamura,(DVM, PhD), Department of Veterinary Medicine, Nihon University