KBM EV Pure (Serum-free medium for Exosome production)
efficiently produce exosomes.This Product is a medium for producing exosome derived from mesenchymal stem cell.
After mesenchymal stem cells are grown in cell culture medium, they can be replaced
with KBM EV Pure to efficiently produce exosomes.
Any type of cell growth medium is acceptable.
Features
- Animal Origin Free(AOF)and Serum Free culture medium.
Lower the risk of infectious diseases caused by materials derived from different animal species. - Low-protein Composition
As purifying exosomes, contamination of proteins derived from the culture medium can be suppressed. - Phenol red free
- Chemically Defined medium
Applicable cell source of exosome
- Canine Adipose-derived MSCs
- Canine Bone marrow-derived MSCs
- Human Adipose-derived MSCs
- Human Bone marrow-derived MSCs etc.
Example of use
-
Seed cells
72 h Culture cells in cell culture medium (serum or serum-free medium)
Replace Medium to KBM EV Pure
24-48 h Maintain cells and produce exosome
24-48 h Maintain cells and produce exosome
Collect culture supernatant solution
Purify exosomes
Data
Immediately after medium replacement
After 48 hours of maintenance
①Purified 9mL of supernatant fluid sampleLane(A) PC(Human-derived)
Lane(B) Culture supernatant (before purification)
Lane2 to 9 Elution fraction#2 to 9 1.0mL
Lane1 and 10 Elution fraction#1 and #10 0.5mL
②Sample 2-10 mixed and concentrated (100 μL collection)Lane(C) Before Concentration （Elution fractions 2-10 mix）
Lane(D) After Concentration （Elution fractions 2-10 mix） Fig. 2：Surface marker expression
Fig. 3：Particle concentration and size distribution of EVs
Data Provided by：Prof. Kazuya Edamura,(DVM, PhD), Department of Veterinary Medicine, Nihon University
Product List
KBM EV Pure (Serum-free medium for Exosome production)
| Product name | Catalog Number |
|---|---|
| KBM EV Pure | KJN-16050700 |
| Documents & Links for KBM EV Pure | |
| Datasheet | KBM EV Pure Datasheet |
| Documents & Links for KBM EV Pure | |
| Datasheet | KBM EV Pure Datasheet |