iTAg Tetramer/PE - H-2 Db gp100 (KVPRNQDWL)

MBL Life Sciences

SKU: MBL-TS-M505-1

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 151

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: PE
• Peptide Sequence: KVPRNQDWL
• Class: Class I
• Sequence Location: 25-33
• MHC Allele: H-2D^b

Citations for iTAg Tetramer/PE - H-2 Db gp100 (KVPRNQDWL) – 1 Found

Takada, Kensuke; Van Laethem, Francois; Xing, Yan; Akane, Kazuyuki; Suzuki, Haruhiko; Murata, Shigeo; Tanaka, Keiji; Jameson, Stephen C; Singer, Alfred; Takahama, Yousuke. TCR affinity for thymoproteasome-dependent positively selecting peptides conditions antigen responsiveness in CD8(+) T cells. Nature Immunology. 2015;16(10):1069-76.