iTAg Tetramer/APC - H-2 Db gp100 (KVPRNQDWL)

MBL Life Sciences

SKU: MBL-TS-M505-2

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 152

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: APC
• Peptide Sequence: KVPRNQDWL
• Class: Class I
• Sequence Location: 25-33
• MHC Allele: H-2D^b

Citations for iTAg Tetramer/APC - H-2 Db gp100 (KVPRNQDWL) – 1 Found

Ouchi, Yasuo; Patil, Ashwini; Tamura, Yusuke; Nishimasu, Hiroshi; Negishi, Aina; Paul, Sudip Kumar; Takemura, Naoki; Satoh, Takeshi; Kimura, Yasumasa; Kurachi, Makoto; Nureki, Osamu; Nakai, Kenta; Kiyono, Hiroshi; Uematsu, Satoshi. Generation of tumor antigen-specific murine CD8+ T cells with enhanced anti-tumor activity via highly efficient CRISPR/Cas9 genome editing. International Immunology. 2018;30(4):141-154.