IMMUNO SHOT Reagent 1

Cosmo Bio

Catalog No.: CSR-IS-001-250

IMMUNO SHOT is an enhancer of antigen-antibody reaction. In Western blotting and ELISA, researchers often experience a weak signal or high background. IMMUNO SHOT improves these problems it as antibody diluents. Due to the principle ofworking mechanism, IMMUNO SHOT can be used in many assay systems that use antigen-antibody reaction.

How IMMUNO SHOT Works

IMMUNO SHOT contains a polymer which, by changing the physicochemical properties of antigen and antibody, enhances the mutual accessibility, and facilitate the specific reaction. The other ingredient, protein, reduces non-specific binding of antibody. Thus, IMMUNO SHOT enhances the antigen-antibody reaction while reduces background

Applications

 IMMUNO SHOT is consisted of Reagent 1 and Reagent 2.Use Reagent 1 for the dilution of the 1st antibody. Use Reagent 2 for the dilution of the 2nd antibody.No change is required for the other assay protocol.

In some assay systems, only one antibodyis used.Forexample some ELISA uses only one enzyme-conjugated antibody.In such case, try using Reagent 2 for the antibody dilution.In some cases, however, Reagent 1 gives better result.

IMMUNO SHOT has been successfully used in Western blotting, antibody sandwich ELISA with either the 1st or 2nd antibody-labeled type, antigen sandwich ELISA

Features and Advantages

 1. High signal with low background IMMUNO SHOT enhances the antigen-antibody reaction. Comparing with the method using detergent-containing buffer, several to over 10-fold stronger signal can be obtained while the background level is low. Thus, you can get much higher S/N ratio than usual method.

2. Effective for saving antibody usage and time of reaction time Because higher signal can be obtained using IMMUNO SHOT, you can reduce the amount of antibody used and the time required for reactions.

3. Can be used for many reactions IMMUNO SHOT can be used not only for Western blotting and ELISA, but also forother assay systems using antigen-antibody reactions. In addition, IMMUNO SHOT does not affect activities of HRP (horse radish peroxidase) or AP (alkaline phosphatase), and can be used for assay systems using these enzymes.

4. Easy to use IMMUNO SHOT is formulatedas to Ready to Use. Just exchange your dilution buffer of antibodies to the solutions of IMMUNO SHOT.

Characteristics of Composition

Solution F: 

The solution is designed to maximize the background-reducing activity. Ideal for observing fine structuresbyusing antibodies with high specificity and sensitivity.

Solution M:

The solution shows characteristics of middle of F and S. Ideal for initial try.

Solution S:

The solution is designed to maximize the specific signal enhancing activity. Idealfor observing strong signal by using antibodies with less specificity and sensitivity. This solution has less ability to reduce background.

(Caution: the above description shows general characteristics of the solutions, and the results you get maybe different depending on the antibody and antigen you are interested in.)

Citations for IMMUNO SHOT Reagent 1 – 10 Found

Kishikawa, Takahiro; Otsuka, Motoyuki; Yoshikawa, Takeshi; Ohno, Motoko; Ijichi, Hideaki; Koike, Kazuhiko. Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1. Nature Communications. 2016;7( 27667193):13006. 

Zhang, Jian; Xie, Jindou; Huang, Ji; Liu, Xiangyang; Xu, Ruihong; Tholen, Jonas; Galej, Wojciech P; Tong, Liang; Manley, James L; Liu, Zhaoqi. Characterization of the SF3B1-SUGP1 interface reveals how numerous cancer mutations cause mRNA missplicing. Genes & Development. 2023;37(21-24):968-983. 

Kinuhata, Toshiki; Sato, Keita; Bando, Tetsuya; Mito, Taro; Miyaishi, Satoru; Nohno, Tsutomu; Ohuchi, Hideyo. Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium. Journal Of Developmental Biology. 2022;10(4) 

Kobayashi, Yukihiro; Kuriyama, Takuma; Nakagawara, Ryoko; Aihara, Michiko; Hamada-Sato, Naoko. Allergy to fish collagen: Thermostability of collagen and IgE reactivity of patients' sera with extracts of 11 species of bony and cartilaginous fish. Allergology International : Official Journal Of The Japanese Society Of Allergology. 2016;65(4):450-458. 

Suzuki, Masataka; Sasabe, Jumpei; Miyoshi, Yurika; Kuwasako, Kanako; Muto, Yutaka; Hamase, Kenji; Matsuoka, Masaaki; Imanishi, Nobuaki; Aiso, Sadakazu. Glycolytic flux controls D-serine synthesis through glyceraldehyde-3-phosphate dehydrogenase in astrocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2015;112(17):E2217-24. 

Iida, Reiko; Ueki, Misuzu; Fujihara, Junko; Takeshita, Haruo; Kimura-Kataoka, Kaori; Yasuda, Toshihiro. Three Nonsynonymous Single Nucleotide Polymorphisms in the RhitH Gene Cause Reduction of the Repression Activity That Leads to Upregulation of M-LPH, a Participant in Mitochondrial Function. Bioresearch Open Access. 2013;2(6):440-7. 

Toda, Katsumi; Ono, Masafumi; Yuhki, Koh-ichi; Ushikubi, Fumitaka; Saibara, Toshiji. 17β-Estradiol is critical for the preovulatory induction of prostaglandin E(2) synthesis in mice. Molecular And Cellular Endocrinology. 2012;362(1-2):176-82. 

Sato, Fuyuki; Kawamura, Haruka; Wu, Yunyan; Sato, Hiroyasu; Jin, Daiki; Bhawal, Ujjal Kumar; Kawamoto, Takeshi; Fujimoto, Katsumi; Noshiro, Mitsuhide; Seino, Hiroko; Morohashi, Satomo; Kato, Yukio; Kijima, Hiroshi. The basic helix-loop-helix transcription factor DEC2 inhibits TGF-β-induced tumor progression in human pancreatic cancer BxPC-3 cells. International Journal Of Molecular Medicine. 2012;30(3):495-501. 

Wu, Yunyan; Sato, Fuyuki; Yamada, Toshiyuki; Bhawal, Ujjal Kumar; Kawamoto, Takeshi; Fujimoto, Katsumi; Noshiro, Mitsuhide; Seino, Hiroko; Morohashi, Satoko; Hakamada, Kenichi; Abiko, Yoshimitsu; Kato, Yukio; Kijima, Hiroshi. The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer. International Journal Of Oncology. 2012;41(4):1337-46. 

Li, Jun; Hakata, Yoshiyuki; Takeda, Eri; Liu, Qingping; Iwatani, Yasumasa; Kozak, Christine A; Miyazawa, Masaaki. Two genetic determinants acquired late in Mus evolution regulate the inclusion of exon 5, which alters mouse APOBEC3 translation efficiency. Plos Pathogens. 2012;8(1):e1002478.