Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α . Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human HIF-1α . Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 1083 nm. The OD value is proportional to the concentration of Human HIF-1α .You can calculate the concentration of Human HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Assay Principle: Sandwich
• Assay Time: 2.5H
• Measurement Range: 62.5-4000pg/mL
• Sensitivity: 37.5 pg/mL
• Sample Type: Tissue homogenate, Cell lysate
• Sample Volume: 25 uL