HLA-A*02:01 Insulin Tetramer-ALWGPDPAAA-PE

MBL Life Sciences

Catalog No.: MBL-TS-M248-1

Product Description

T lymphocytes play a central role in immune system function. Total T cell and T cell subset counts are measured by detection of various cell surface molecules. Enumeration of CD8* antigen-specific T cells requires cognate recognition of the T cell receptor (TCR) by a class | MHC/peptide complex. This can be done using class I MHC Tetramers which are composed of a complex of four HLA class I molecules each bound to the specific peptide and conjugated with a fluorescent protein. Thus, T-Select MHC Tetramer assays allow quantitation of the total T cell population specific for a given peptide complexed with a particular MHC molecule. Furthermore, since binding does not depend on functional pathways, this population includes all specific CD8+ T cells regardless of functional status. Measurements may be performed in whole blood or isolated lymphocyte/mononuclear cell preparations. Specific cell staining is accomplished by incubating the sample with the T-Select MHC Tetramer reagent, then washing away excess Tetramer. The number of Tetramer positive lymphocytes is then determined by flow cytometry.

Product Specifications

• Immunogen: Insulin
• Peptide Sequence: ALWGPDPAAA
• MHC Allele: A*02:01