Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 206
Product Specifications
Application
FC
Reactivity
Mouse
Conjugation
APC
Immunogen
islet-specific glucose-6-phosphatase catalytic subunit related protein
Peptide Sequence
IGRP
Class
Class I
Sequence Location
206-214
MHC Allele
H-2Kd
Documents & Links for H-2Kd IGRP Tetramer-VYLKTNVFL-APC