H-2Kd Erk1 Tetramer-QYIHSANVL-PE

MBL Life Sciences

Catalog No.: MBL-TS-M545-1

Product Description

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones.For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 255

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: PE
• Immunogen: Erk2 K136Q
• Peptide Sequence: QYIHSANVL
• Class: Class I
• Sequence Location: 136-144
• MHC Allele: H-2K^d

Citations for H-2Kd Erk1 Tetramer-QYIHSANVL-PE – 2 Found

Nishikawa, Hiroyoshi; Kato, Takuma; Hirayama, Michiko; Orito, Yuki; Sato, Eiichi; Harada, Naozumi; Gnjatic, Sacha; Old, Lloyd J; Shiku, Hiroshi. Regulatory T cell-resistant CD8+ T cells induced by glucocorticoid-induced tumor necrosis factor receptor signaling. Cancer Research. 2008;68(14):5948-54. 

Ikeda, H; Ohta, N; Furukawa, K; Miyazaki, H; Wang, L; Kuribayashi, K; Old, L J; Shiku, H. Mutated mitogen-activated protein kinase: a tumor rejection antigen of mouse sarcoma. Proceedings Of The National Academy Of Sciences Of The United States Of America. 1997;94(12):6375-9.