
Product Description
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 149Product Specifications | |
Application | FC |
Reactivity | Mouse |
Conjugation | PE |
Peptide Sequence | β-galactosidase |
Class | Class I |
Sequence Location | 96-103 |
MHC Allele | H-2Kb |
Documents & Links for H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE | |
Datasheet | H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE Datasheet |
Documents & Links for H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE | |
Datasheet | H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE Datasheet |
Citations for H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE – 1 Found |
Hemmi, Masahisa; Tachibana, Masashi; Fujimoto, Natsuki; Shoji, Masaki; Sakurai, Fuminori; Kobiyama, Kouji; Ishii, Ken J; Akira, Shizuo; Mizuguchi, Hiroyuki. T Helper 17 Promotes Induction of Antigen-Specific Gut-Mucosal Cytotoxic T Lymphocytes following Adenovirus Vector Vaccination. Frontiers In Immunology. 8:1456. PubMed |