H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE

MBL Life Sciences

Catalog No.: MBL-TS-M501-1

Product Description

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 149

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: PE
• Immunogen: β-galactosidase
• Peptide Sequence: DAPIYTNV
• Class: Class I
• Sequence Location: 96-103
• MHC Allele: H-2K^b

Citations for H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE – 1 Found

Hemmi, Masahisa; Tachibana, Masashi; Fujimoto, Natsuki; Shoji, Masaki; Sakurai, Fuminori; Kobiyama, Kouji; Ishii, Ken J; Akira, Shizuo; Mizuguchi, Hiroyuki. T Helper 17 Promotes Induction of Antigen-Specific Gut-Mucosal Cytotoxic T Lymphocytes following Adenovirus Vector Vaccination. Frontiers In Immunology. 8:1456.