H-2Db gp100 Tetramer-EGSRNQDWL-APC

MBL Life Sciences

Catalog No.: MBL-TS-M546-2

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 258

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: APC
• Peptide Sequence: EGSRNQDWL
• Class: Class I
• Sequence Location: 25-33
• MHC Allele: H-2D^b