Principle: The reducing terminal of sugar is labeled with 4-aminobenzoic acid methyl ester (ABEE) through reductive amination. ABEE-labeled sugar can be applied to HPLC analyses using GlyScope Honenpak C18 (for ABEE Labeling Kit) (Cat. No. JOM-J715) due to its absorbance ability of fluorescence or ultraviolet.* This kit is not suitable for analyses of the sugars which do not have aldehyde group (e.g. sugar alcohol (xylitol, erythritol etc) or ketose (fructose)).
Note: 1 bottle is for 20 tests. All reagents should be stored at -20C. Do not store the bottle once it's opened. One bottle should be used for one assay. There is no problem for usage if the reagents freeze.
Required Materials and Equipment, Not Provided: 1.Trifluoroacetic acid (TFA) 2.2- propanol (isopropyl alcohol) 3.Chloroform 4.Acetonitrile (for HPLC) 5.Standard sugars
