Fluoppi Ver.2: Ash-Red (Ash-MNL/MCL + Monti-Red-MNL/MCL)

MBL Life Sciences

Catalog No.: MBL-AM-8012M

Product Description

Fluoppi is a technology providing an easy way to visualize protein-protein interactions (PPIs) with a high signal to noise ratio. It employs an oligomeric assembly helper tag (Ash-tag) and a tetrameric fluorescent protein tag (FP-tag) to create detectable fluorescent foci when there are interactions between two proteins fused to the tags. By way of example, genetic fusion of protein X with FP-tag, and Y with Ash-tag creates a tetrameric fluorescent fusion protein X-FP and an oligomeric fusion protein Y-Ash respectively. Because each fusion protein has multiple Xs or Ys, interaction between X and Y causes large lattice like complexes where the fluorescence by X-FP is concentrated and detectable as fluorescent foci. Anti-Ash-tag monoclonal antibody (mAb) recognizes the joint region between Ash-tag and flexible linker . Fluoppi Ver.2 : Ash-Red contains an additional amino acid at the C terminal of Ash-tag expressed by pAsh-MNL, which make it possible to be recognized by the mAbFluoppi Ver.2 : Ash-Red includes 4 expression plasmids as listed in section 2. A tetrameric red fluorescent protein, Monti-Red, is employed as a FP-tag as describe

Citations for Fluoppi Ver.2: Ash-Red (Ash-MNL/MCL + Monti-Red-MNL/MCL) – 6 Found

Koyano, Fumika; Okatsu, Kei; Kosako, Hidetaka; Tamura, Yasushi; Go, Etsu; Kimura, Mayumi; Kimura, Yoko; Tsuchiya, Hikaru; Yoshihara, Hidehito; Hirokawa, Takatsugu; Endo, Toshiya; Fon, Edward A; Trempe, Jean-François; Saeki, Yasushi; Tanaka, Keiji; Matsuda, Noriyuki. Ubiquitin is phosphorylated by PINK1 to activate parkin. Nature. 2014;510(7503):162-6. 

Yamano, Koji; Queliconi, Bruno B; Koyano, Fumika; Saeki, Yasushi; Hirokawa, Takatsugu; Tanaka, Keiji; Matsuda, Noriyuki. Site-specific Interaction Mapping of Phosphorylated Ubiquitin to Uncover Parkin Activation. The Journal Of Biological Chemistry. 2015;290(42):25199-211. 

Asamitsu, Kaori; Omagari, Katsumi; Okuda, Tomoya; Hibi, Yurina; Okamoto, Takashi. Quantification of the HIV transcriptional activator complex in live cells by image-based protein-protein interaction analysis. Genes To Cells : Devoted To Molecular & Cellular Mechanisms. 2016;21(7):706-16. 

Watanabe, Taku; Seki, Tatsuya; Fukano, Takashi; Sakaue-Sawano, Asako; Karasawa, Satoshi; Kubota, Misaki; Kurokawa, Hiroshi; Inoue, Ken; Akatsuka, Junichi; Miyawaki, Atsushi. Genetic visualization of protein interactions harnessing liquid phase transitions. Scientific Reports. 2017;7:46380. 

Saito, Ken; Iioka, Hidekazu; Maruyama, Satoshi; Sumardika, I Wayan; Sakaguchi, Masakiyo; Kondo, Eisaku. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Neoplasia (New York, N.y.). 2019;21(12):1121-1132. 

Yoshikawa, Masaru; Yoshii, Tatsuyuki; Ikuta, Masahiro; Tsukiji, Shinya. Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. Journal Of The American Chemical Society. 2021;143(17):6434-6446.