Fecal Mucin Assay Kit

Cosmo Bio

Catalog No.: CSR-FFA-MU-K01

Background

Mucins are a family of heavily glycosylated, high molecular weight (1000-10,000 kDa) proteins rich in threonine, serine and hydroxyproline. The Fecal Mucin Assay Kit leverages the reducing end of GalNAc sugar chains frequently linked by post-translational O-glycosylation to these residues to fluorescently quantitate mucin content in feces.

Mucins are a family of heavily glycosylated, high molecular weight (1000-10000 kDa) proteins that are the main components of the mucosa, including in saliva, tears, gastric fluid and enteric fluid. Mucins are involved in gut mucosal barrier function, stopping the translocation of pathogens and toxins into blood vessels beyond the intestinal wall. Heterogeneous sugar chain components of mucins contribute to their diverse structures and binding targets, including specific proteins derived from viruses and bacteria.

Assay principle

Mucins are a family of heavily glycosylated, high molecular weight (1000-10000 kDa) proteins. Mucin domains within their protein cores are rich in threonine, serine and hydroxyproline. The reducing end of GalNAc sugar chains are frequently linked to threonine, serine and hydroxyproline by post-translational O-glycosylation. O-glycosidically linked oligosaccharide chains are β-eliminated by diluted alkali to form reducing ends of sugar chains. Reducing carbohydrates are subsequently fluorescently labeled at high temperature to produce an intensely fluorescent condensate.

Applications

• Intestinal flora research
• Food research
• Agricultural research
• Functional food development

Citations for Fecal Mucin Assay Kit – 40 Found

Niibo, M; Shirouchi, B; Umegatani, M; Morita, Y; Ogawa, A; Sakai, F; Kadooka, Y; Sato, M. Probiotic Lactobacillus gasseri SBT2055 improves insulin secretion in a diabetic rat model. Journal Of Dairy Science. 2019;102(2):997-1006. 
- Comments: Cosmo Bio’s Fecal Mucin Assay Kit was used in a study showing that canna starch supplementation in a murine food allergy model suppresses anaphylactic symptoms by improving the intestinal environment and reducing allergen uptake by increasing intestinal barrier function.

Kasahara, Naoya; Teratani, Takumi; Yokota, Shinichiro; Sakuma, Yasunaru; Sasanuma, Hideki; Fujimoto, Yasuhiro; Ijichi, Tetsuo; Urahashi, Taizen; Yoshitomi, Hideyuki; Kitayama, Joji; Sata, Naohiro. Dietary polyamines promote intestinal adaptation in an experimental model of short bowel syndrome. Scientific Reports. 2024;14(1):4605. 
- Comments: Cosmo Bio’s Fecal Mucin Assay Kit was used in a study showing that dietary polyamines promote intestinal adaptation in a rat SBS model via a GLP-2-independent mechanism and enhance autophagy as well as suppress lipogenic gene expression in the liver.

Du, Yao; Kusama, Kanta; Hama, Koki; Chen, Xinyue; Tahara, Yu; Kajiwara, Susumu; Shibata, Shigenobu; Orihara, Kanami. Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease. International Journal Of Molecular Sciences. 2024;25(5) 
- Comments: Cosmo Bio’s Fecal Mucin Assay Kit was used in a study showing that inulin, a natural prebiotic, can improve stress-recurrent inflammatory bowel disease symptoms by modulating microbiota composition, reducing inflammation, and alleviating ER stress.

Koida, Ayaka; Tanaka, Mamoru; Kosaka, Rina; Okuda, Shoei; Takei, Shiro; Ota, Suzuno; Yokoyama, Sayaka; Miyake, Kaho; Watanabe, Hiroyuki. Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice. Biomolecules. 2024;14(2) 

Tian, Yuan; Rimal, Bipin; Bisanz, Jordan E; Gui, Wei; Wolfe, Trenton M; Koo, Imhoi; Murray, Iain A; Nettleford, Shaneice K; Yokoyama, Shigetoshi; Dong, Fangcong; Koshkin, Sergei; Prabhu, K Sandeep; Turnbaugh, Peter J; Walk, Seth T; Perdew, Gary H; Patterson, Andrew D. Effects of Early Life Exposures to the Aryl Hydrocarbon Receptor Ligand TCDF on Gut Microbiota and Host Metabolic Homeostasis in C57BL/6J Mice. Environmental Health Perspectives. 2024;132(8):87005. 

Murakami, Yuki; Hosomi, Ryota; Tanaka, Genki; Murakami, Hirokazu; Kanto, Ayaka; Kimura, Takahiro; Imamura, Yukio; Yoshida, Munehiro; Fukunaga, Kenji. Fish (Alaska Pollock) protein intake attenuates age-related short-term memory decline through gut microbiota modulation. Scientific Reports. 2026;16(1) 

Fujiwara, Shin; Park, Jonguk; Takeda, Mariko; Miyatake, Takumi; Saito, Yoshie; Makino, Seiya; Kim, Yun-Gi. Sialic acid-responsive Parabacteroides is linked to gut barrier integrity in older adults. Gut Microbes. 2026;18(1):2627093. 

Hamaguchi, Tomonari; Gibo, Noriaki; Ohara, Misuzu; Ito, Mikako; Ogura, Tomoyuki; Takeda, Jun-Ichi; Nishiwaki, Hiroshi; Zhao, Fei; Kinoshita-Daitoku, Ryo; Hattori, Masashi; Nonogaki, Koji; Maeda, Tetsuya; Kashihara, Kenichi; Tsuboi, Yoshio; Hirayama, Masaaki; Fujishiro, Mitsuhiro; Kawashima, Hiroki; Ohno, Kinji. Bacterial constipation: Mucin-degrading intestinal commensal bacteria cause constipation. Gut Microbes. 2026;18(1):2596809. 

Yokoyama, Sayaka; Kikuchi, Amane; Takahashi, Hideaki; Ushimaru, Hinako; Yamaguchi, Hibiki; Yamada, Chikako; Fujiki, Kotoyo; Kozai, Hana; Ota, Suzuno; Fujii, Tadashi; Hirooka, Yoshiki; Tochio, Takumi; Tanaka, Mamoru. Modulation of the Gut Microbiota by Nopalea cochenillifera (Prickly Pear Cactus) Contributes to Improved Lipid Metabolism and Immune Function. Nutrients. 2025;17(17) 

Yokoyama, Sayaka; Kozai, Hana; Kikuchi, Amane; Ota, Suzuno; Horibe, Takanori; Tanaka, Mamoru. Dietary supplementation with Nopalea cochenillifera enhances fecal mucin production and modulates serum immunoglobulin levels in a dose- and time-dependent manner in BALB/c mice. Drug Discoveries & Therapeutics. 2025;19(2):96-102. 

Kato, Miyuna; Goto, Kana; Kani, Koudai; Igarashi, Naoya; Kasai, Kaichi; Tada, Yuki; Yoshimoto, Yousei; Watanabe, Yasuharu; Honda, Hiroe; Ichimura-Shimizu, Mayuko; Watanabe, Shiro; Tsuneyama, Koichi; Furusawa, Yukihiro; Nagai, Yoshinori. Cholic Acid-Induced Akkermansia Expansion and FGF-15 Upregulation Improve Diabetes While Exacerbating Steatohepatitis in TSOD Mice. Genes To Cells : Devoted To Molecular & Cellular Mechanisms. 2025;30(6):e70069. 

Bøgwald, Isak; Pedersen, Alice Marie; Dias, Jorge; Wubshet, Sileshi Gizachew; Eilertsen, Karl-Erik. Dietary Protein Hydrolysate From Calanus finmarchicus Reduces Oxidative Stress and Increases Intestinal Health in European Sea Bass (Dicentrarchus labrax) Juveniles. Aquaculture Nutrition. 2025:5531437. 

Oikawa, Daiki; Ohira, Hideo; Aoki, Yuichi; Kurokawa, Yoichi; Omura, Ayano; Kiyomoto, Kunio; Fujioka, Yoshio; Nakayama, Toru. Oral supplementation with sesaminol, a sesame-related lignan, ameliorates ethanol-induced dysbiosis of the gut microbiota and increases the gut luminal short-chain fatty acid concentrations of mice. The Journal Of Nutritional Biochemistry. 2025;146:110052. 

Houdelet, Camille; Gonçalves, Ana Teresa; Dias, Jorge; Rawling, Mark; Leclercq, Eric. Dose-dependent benefits of dietary yeast cell wall supplementation on the growth performance and stress resilience of the European seabass (Dicentrarchus labrax). Fish Physiology And Biochemistry. 2025;51(6):199. 

Du, Yao; Kusama, Kanta; Hama, Koki; Chen, Xinyue; Tahara, Yu; Kajiwara, Susumu; Shibata, Shigenobu; Orihara, Kanami. Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease. International Journal Of Molecular Sciences. 2024;25(5) 

Kasahara, Naoya; Teratani, Takumi; Yokota, Shinichiro; Sakuma, Yasunaru; Sasanuma, Hideki; Fujimoto, Yasuhiro; Ijichi, Tetsuo; Urahashi, Taizen; Yoshitomi, Hideyuki; Kitayama, Joji; Sata, Naohiro. Dietary polyamines promote intestinal adaptation in an experimental model of short bowel syndrome. Scientific Reports. 2024;14(1):4605. 

Kozai, Hana; Watanabe, Chihiro; Kosaka, Rina; Aoki, Takafumi; Hamada, Hiroki; Kawashima, Masato; Kono, Takumi; Akagi, Kosuke; Kremenik, Michael J; Yano, Hiromi; Tanaka, Mamoru; Oyanagi, Eri. Nopalea cochenillifera Regulates the Immune Response and Gut Microbiota in Mice. Nutrients. 2024;16(24) 

Mishra, Sidharth P; Jain, Shalini; Wang, Bo; Wang, Shaohua; Miller, Brandi C; Lee, Jea Y; Borlongan, Cesar V; Jiang, Lin; Pollak, Julie; Taraphder, Subhash; Layden, Brian T; Rane, Sushil G; Yadav, Hariom. Abnormalities in microbiota/butyrate/FFAR3 signaling in aging gut impair brain function. Jci Insight. 2024;9(3) 

Koida, Ayaka; Tanaka, Mamoru; Kosaka, Rina; Okuda, Shoei; Takei, Shiro; Ota, Suzuno; Yokoyama, Sayaka; Miyake, Kaho; Watanabe, Hiroyuki. Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice. Biomolecules. 2024;14(2) 

Tanaka, Mamoru; Koida, Ayaka; Miyazaki, Akira; Tabata, Kazushi; Takei, Yuichiro; Tanimoto, Yoshihumi; Kawamura, Mami; Tsuzuki, Masafumi; Takahashi, Haruka; Yano, Tetsu; Watanabe, Hiroyuki. Canna starch improves immune functions and the intestinal environment in mice. Bioscience Of Microbiota, Food And Health. 42(2):131-137. 

Matsumoto, Kenji; Sawano, Honoka; Otsubo, Marina; Yui, Asuka. Comparison of the Effects of 3 Forms of Soluble Dietary Fiber on the Production of IgA in BALB/cAJcl and BALB/cAJcl-nu/nu Mice. The Journal Of Nutrition. 2023;153(5):1618-1626. 

Asao, Kazunobu; Hashida, Noriyasu; Motooka, Daisuke; Tsukamoto, Teruhisa; Nakamura, Shota; Maruyama, Kazuichi; Nishida, Kohji. Fungal dysbiosis and decreased tear mucin at the conjunctiva in patients with conjunctival mucosa-associated lymphoid tissue lymphoma. Bmj Open Ophthalmology. 2023;8(1) 

Nakano, Hironobu; Setoguchi, Sho; Kawano, Kuniaki; Miyagawa, Hiroshi; Sakao, Kozue; Hou, De-Xing. Effects of Amazake Produced with Different Aspergillus on Gut Barrier and Microbiota. Foods (Basel, Switzerland). 2023;12(13) 

Zhou, Xiaoliang; Chakraborty, Debopriya; Murray, Iain A; Coslo, Denise; Kehs, Zoe; Vijay, Anitha; Ton, Carolyn; Desai, Dhimant; Amin, Shantu G; Patterson, Andrew D; Perdew, Gary H. Aryl Hydrocarbon Receptor Activation Coordinates Mouse Small Intestinal Epithelial Cell Programming. Laboratory Investigation; A Journal Of Technical Methods And Pathology. 2023;103(2):100012. 

Watanabe, Yoshiyuki; Fujisaka, Shiho; Morinaga, Yoshitomo; Watanabe, Shiro; Nawaz, Allah; Hatta, Hideki; Kado, Tomonobu; Nishimura, Ayumi; Bilal, Muhammad; Aslam, Muhammad Rahil; Honda, Keiko; Nakagawa, Yoshimi; Softic, Samir; Hirabayashi, Kenichi; Nakagawa, Takashi; Nagai, Yoshinori; Tobe, Kazuyuki. Isoxanthohumol improves obesity and glucose metabolism via inhibiting intestinal lipid absorption with a bloom of Akkermansia muciniphila in mice. Molecular Metabolism. 2023;77:101797. 

Ishibashi, Riko; Furusawa, Yukihiro; Honda, Hiroe; Watanabe, Yasuharu; Fujisaka, Shiho; Nishikawa, Miyu; Ikushiro, Shinichi; Kurihara, Shin; Tabuchi, Yoshiaki; Tobe, Kazuyuki; Takatsu, Kiyoshi; Nagai, Yoshinori. Isoliquiritigenin Attenuates Adipose Tissue Inflammation and Metabolic Syndrome by Modifying Gut Bacteria Composition in Mice. Molecular Nutrition & Food Research. 2022;66(10):e2101119. 

Shikano, Riho; Ohno, Koichi; Nagahara, Takuro; Nagao, Itsuma; Toyoda, Hiroto; Nakagawa, Taisuke; Goto-Koshino, Yuko; Chambers, James K; Tomiyasu, Hirotaka; Tsujimoto, Hajime. Effects of proportions of carbohydrates and fats in diets on mucin concentration and bile composition in gallbladder of dogs. The Journal Of Veterinary Medical Science. 2022;84(11):1465-1468. 

Taira, Toshio; Yamaguchi, Sayori; Takahashi, Azusa; Okazaki, Yukako; Yamaguchi, Akihiro; Sakaguchi, Hirohide; Chiji, Hideyuki. Dietary polyphenols increase fecal mucin and immunoglobulin A and ameliorate the disturbance in gut microbiota caused by a high fat diet. Journal Of Clinical Biochemistry And Nutrition. 2015;57(3):212-6. 

Anhê, Fernando F; Nachbar, Renato T; Varin, Thibault V; Vilela, Vanessa; Dudonné, Stéphanie; Pilon, Geneviève; Fournier, Maryse; Lecours, Marc-André; Desjardins, Yves; Roy, Denis; Levy, Emile; Marette, André. A polyphenol-rich cranberry extract reverses insulin resistance and hepatic steatosis independently of body weight loss. Molecular Metabolism. 2017;6(12):1563-1573. 

Nirmagustina, Dwi Eva; Yang, Yongshou; Kumrungsee, Thanutchaporn; Yanaka, Noriyuki; Kato, Norihisa. Gender Difference and Dietary Supplemental Vitamin B(6): Impact on Colon Luminal Environment. Journal Of Nutritional Science And Vitaminology. 64(2):116-128. 

Mezhibovsky, Esther; Wu, Yue; Bawagan, Fiona G; Tveter, Kevin M; Szeto, Samantha; Roopchand, Diana. Impact of grape polyphenols on Akkermansia muciniphila and the gut barrier. Aims Microbiology. 8(4):544-565. 

Amorim Neto, Dionísio Pedro; Bosque, Beatriz Pelegrini; Pereira de Godoy, João Vitor; Rodrigues, Paulla Vieira; Meneses, Dario Donoso; Tostes, Katiane; Costa Tonoli, Celisa Caldana; Faustino de Carvalho, Hernandes; González-Billault, Christian; de Castro Fonseca, Matheus. Akkermansia muciniphila induces mitochondrial calcium overload and α -synuclein aggregation in an enteroendocrine cell line. Iscience. 2022;25(3):103908. 

Higashimura, Yasuki; Hirabayashi, Misaki; Nishikawa, Hitomi; Inoue, Ryo; Nagai, Emiko; Matsumoto, Kenji; Enomoto, Toshiki; Mizushima, Katsura; Takagi, Tomohisa; Naito, Yuji. Dietary intake of yacon roots (Smallanthus sonchifolius) affects gut microbiota and fecal mucin and prevents intestinal inflammation in mice. Journal Of Clinical Biochemistry And Nutrition. 2021;69(3):272-279. 

Ju, Tingting; Kennelly, John P; Jacobs, René L; Willing, Benjamin P. Insufficient dietary choline aggravates disease severity in a mouse model of Citrobacter rodentium-induced colitis. The British Journal Of Nutrition. 2021;125(1):50-61. 

Kawakami, Shinpei; Ito, Ryouichi; Maruki-Uchida, Hiroko; Kamei, Asuka; Yasuoka, Akihito; Toyoda, Tsudoi; Ishijima, Tomoko; Nishimura, Eisaku; Morita, Minoru; Sai, Masahiko; Abe, Keiko; Okada, Shinji. Intake of a Mixture of Sake Cake and Rice Malt Increases Mucin Levels and Changes in Intestinal Microbiota in Mice. Nutrients. 2020;12(2) 

Kawashima, Rei; Tamaki, Shun; Kawakami, Fumitaka; Maekawa, Tatsunori; Ichikawa, Takafumi. Histamine H(2)-Receptor Antagonists Improve Non-Steroidal Anti-Inflammatory Drug-Induced Intestinal Dysbiosis. International Journal Of Molecular Sciences. 2020;21(21) 

Ansia, I; Drackley, J K. Technical note: Evaluation of 3 methods to determine mucin protein concentration in ileal digesta of young preweaning calves. Journal Of Dairy Science. 2020;103(7):6250-6257. 

Takahashi, Masanobu; Takahashi, Kento; Abe, Sunao; Yamada, Kosuke; Suzuki, Manami; Masahisa, Mai; Endo, Mari; Abe, Keiko; Inoue, Ryo; Hoshi, Hiroko. Improvement of Psoriasis by Alteration of the Gut Environment by Oral Administration of Fucoidan from Cladosiphon Okamuranus. Marine Drugs. 2020;18(3) 

Fujisaka, Shiho; Usui, Isao; Nawaz, Allah; Igarashi, Yoshiko; Okabe, Keisuke; Furusawa, Yukihiro; Watanabe, Shiro; Yamamoto, Seiji; Sasahara, Masakiyo; Watanabe, Yoshiyuki; Nagai, Yoshinori; Yagi, Kunimasa; Nakagawa, Takashi; Tobe, Kazuyuki. Bofutsushosan improves gut barrier function with a bloom of Akkermansia muciniphila and improves glucose metabolism in mice with diet-induced obesity. Scientific Reports. 2020;10(1):5544. 

Ayumu Horinouchi et., al. . Intestinal immunomodulatory activity of indigestible glucan in mice and its utilization by intestinal bacteria in vitro. Journal of Functional Foods . 2021;87