Fast Step VD3 (Vitamin D3) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VD3 . Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to VD3 . Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: All
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 6.25-400 ng/mL
• Sensitivity: 2.14 ng/mL
• Sample Type: Serum, Plasma and Other biological fluids
• Sample Volume: 50 uL