Fast Step VD (Vitamin D) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VD . Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for VD are added to the micro ELISA plate wells. VD in samples (or standards) competes with a fixed amount of VD on the solid phase supporter for sites on the HRP linked detection antibody specific to VD . Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±3 nm. The concentration of VD in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: All
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 6.25-400 ng/mL
• Sensitivity: 2.19 ng/mL
• Sample Type: Serum, Plasma and Other biological fluids
• Sample Volume: 50 uL