Fast Step Rat sCD86 (Soluble Cluster of Differentiation 86) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sCD86 . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Rat sCD86 are added to the micro ELISA plate wells. Rat sCD86 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat sCD86 . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat sCD86 . You can calculate the concentration of Human 96-NT in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Rat
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 15.63-1000pg/mL
• Sensitivity: 8.93 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
• Sample Volume: 50 uL