Fast Step Rat SAA (Serum Amyloid A) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SAA . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Rat SAA are added to the micro ELISA plate wells. Rat SAA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat SAA . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat SAA . You can calculate the concentration of Human 90-NT in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Rat
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 1.57-100pg/mL
• Sensitivity: 0.94 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenate and Other biological samples
• Sample Volume: 50 uL