Fast Step Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1/CD54 . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Rat ICAM-1/CD54 are added to the micro ELISA plate wells. Rat ICAM-1/CD54 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ICAM-1/CD54 . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat ICAM-1/CD54 . You can calculate the concentration of Human 302-NT in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Rat
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 0.31-20ng/mL
• Sensitivity: 0.01 ng/mL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
• Sample Volume: 50 uL