Fast Step Rat HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Rat HIF-1α are added to the micro ELISA plate wells. Rat HIF-1α in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat HIF-1α . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat HIF-1α . You can calculate the concentration of Human 222-NT in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Rat
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 7.82-500pg/mL
• Sensitivity: 4.69 pg/mL
• Sample Type: Tissue homogenate, Cell lysate
• Sample Volume: 50 uL