Fast Step Rat D2D (D-Dimer) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat D2D . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Rat D2D are added to the micro ELISA plate wells. Rat D2D in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat D2D . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat D2D . You can calculate the concentration of Human 340-NT in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Rat
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 78.13-5000 ng/mL
• Sensitivity: 46.88 ng/mL
• Sample Type: Plasma and other biological fluids
• Sample Volume: 50 uL