Fast Step Rat C5a (Complement Component 5a) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5a . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Rat C5a are added to the micro ELISA plate wells. Rat C5a in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat C5a . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat C5a . You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Rat
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 15.63-1000ng/mL
• Sensitivity: 9.38 ng/mL
• Sample Type: Serum, Plasma, Tissue homogenate and Other biological samples
• Sample Volume: 50 uL