Fast Step Mouse VIP (Vasoactive Intestinal Peptide) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse VIP . Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Mouse VIP . Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VIP in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Mouse
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 12.5-800pg/mL
• Sensitivity: 7.5 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenate and Other biological samples
• Sample Volume: 50 uL