Fast Step Mouse F12 (Coagulation Factor Ⅻ) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse F12 . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Mouse F12 are added to the micro ELISA plate wells. Mouse F12 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse F12 . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse F12 . You can calculate the concentration of Mouse F12 in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Mouse
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 62.5-4000pg/mL
• Sensitivity: 12.51 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
• Sample Volume: 50 uL