Fast Step Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sCD40L . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Human sCD40L are added to the micro ELISA plate wells. Human sCD40L in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human sCD40L . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human sCD40L . You can calculate the concentration of Human sCD40L in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Human
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 62.5-4000pg/mL
• Sensitivity: 37.5 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenate and Other biological samples
• Sample Volume: 50 uL