Fast Step Human S100B (S100 Calcium Binding Protein B) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Human S100B are added to the micro ELISA plate wells. Human S100B in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human S100B . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human S100B . You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Human
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 7.81-500pg/mL
• Sensitivity: 3.11 pg/mL
• Sample Type: cerebrospinal fluid, blood, Urine, amniotic fluid
• Sample Volume: 50 uL