Fast Step Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-27/HSPB1 . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Human HSP-27/HSPB1 are added to the micro ELISA plate wells. Human HSP-27/HSPB1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human HSP-27/HSPB1 . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human HSP-27/HSPB1 . You can calculate the concentration of Human 12-NT in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Human
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 0.79-50ng/mL
• Sensitivity: 0.47 ng/mL
• Sample Type: Tissue homogenate and Cell lysate
• Sample Volume: 50 uL