Fast Step Human ET-1 (Endothelin 1) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1 . Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Human ET-1 are added to the micro ELISA plate wells. Human ET-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ET-1 . Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human ET-1 . You can calculate the concentration of Human ET-1 in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: Human
• Assay Principle: Sandwich
• Assay Time: 1.5H
• Measurement Range: 1.25-80pg/mL
• Sensitivity: 0.75 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenate and Other biological samples
• Sample Volume: 50 uL