Fast Step E3 (Estriol) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E3 . Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for E3 are added to the micro ELISA plate wells. E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3 . Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±20 nm. The concentration of E3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: All
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 1.57-100 ng/mL
• Sensitivity: 0.48 ng/mL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
• Sample Volume: 50 uL