Fast Step cAMP (Cyclic Adenosine Monophosphate) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP . Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to cAMP . Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: All
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 312.5-20000 pg/mL
• Sensitivity: 92.5 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
• Sample Volume: 50 uL