Fast Step 5-HT (5-Hydroxytryptamine) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HT . Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 5-HT . Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: All
• Assay Principle: Competitive
• Assay Time: 45min
• Measurement Range: 14.07-900 ng/mL
• Sensitivity: 3.97 ng/mL
• Sample Type: Serum, Plasma, Tissue homogenates
• Sample Volume: 50 uL