EZGlyco® O-Glycan Prep Kit

S-BIO

Catalog No.: SBC-BS-41601Z

Product Description

EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation.

The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis.
The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans.

Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control.
It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products.

EZGlyco O-Glycan Prep Kit Components

• Protocol
• Glycan Release Reagent A
• Glycan Release Reagent B
• Glycan Capturing Beads
• Filter Column
• 2-Aminobezamide
• Reducing Reagent
• Cleanup Column

The kit provides sufficient reagents for 10 tests.

Benefits of the EZGlyco O-Glycan Prep Kit

• O-Glycan Sample Prep in 5 Hours: O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. You can now complete HPLC/LC-MS analysis on the same day as sample prep.
• High Recovery: A combination of the unique O-glycan releasing reagents and the new O-glycan capturing beads enables high recovery of released O-glycan from various glycoproteins.
• Minimize "Peeling": Glycan releasig reagents minimize decomposition (peeling) of O-glycans, resulting in O-glycan characterization with higher accuracy.
• Safe and Easy Operation: With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.