DDDDK tagged Protein PURIFICATION KIT

MBL Life Sciences

SKU: MBL-3325R

Product Description
The ability to isolate and study a purified protein lies at the heart of modern biochemistry.
Researchers in many fields require highly purified, active proteins for studies involving signaling
pathways, enzymology, receptor binding, DNA binding, post-transcriptional modifications, and much
more. Thus, choosing a method of purification is an important aspect in maintaining protein structure
and function.

Recombinant tagged protein purification methods and kits are now widely recognized. The
DDDDK epitope tag (DYKDDDDK) has been widely used as a multi-purpose tag, and
anti-DDDDK-tag antibodies are optimally suited for identifying, detecting, purifying, and monitoring
the expression levels of recombinant DDDDK-tagged proteins.
MBL’s DDDDK-tagged Protein PURIFICATION KIT is designed for the isolation of
DDDDK-tagged protein from cell culture supernatants and cell lysate under neutral pH condition.
Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH
elution can preserve protein activity and native conformation. MBL has developed the
Anti-DDDDK-tag Beads to purify DDDDK-tagged proteins quickly and efficiently. As the Beads can
be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of
DDDDK-tagged proteins from the Beads is achieved by the addition of the DDDDK-tag peptide
(DYKDDDDK). As the DDDDK-tag peptide competes with DDDDK-tagged proteins on the Beads,
the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency
has optimized the simple procedure of this kit.

Kit Components
Components sufficient for conducting 20 times purifications of DDDDK-tagged protein.
1. Anti-DDDDK-tag Beads 25% slurry: 100 L beads in 400 L total volume in PBS with
0.1% ProClin 150 as preservative
2. Elution Peptide DDDDK peptide, 1 mg in 1 mL PBS after reconstitution
3. Spin Columns Sets 20 columns with pre-inserted bottom plugs and top caps
4. Wash Concentrate 10 x concentrate, 6 mL x 2 tubes

Storage
Store for up to 1 year from date of receipt at 2-8°C. Do not freeze.

Product Capacity
The purification capacity of the Anti-DDDDK-tag Beads varies depending upon the DDDDK-tagged
protein.
For example, 1 mL of Anti-DDDDK-tag Beads purified 0.3 mg of DDDDK-tagged protein.