Purification of DDDDK-tagged protein at a neutral pH to maintain the isolated protein's activities and native conformations.
The DDDDK epitope tag (DYKDDDDK) has been widely used as a multi-purpose tag, and anti-DDDDK-tag antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK-tagged proteins.
MBL's DDDDK-tagged Protein PURIFICATION GEL with Elution Peptide is designed for the isolation of DDDDK-tagged protein from cell culture supernatants and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. Therefore, MBL has developed the Anti-DDDDK-tag Gel to purify DDDDK-tagged proteins quickly and efficiently at neutral pH to maintain the activity and conformation of purified proteins. The elution of DDDDK-tagged proteins from the Gel is achieved by the addition of the DDDDK-tag peptide (DYKDDDDK). As the DDDDK-tag peptide competes with DDDDK-tagged proteins on the Gel, the purified proteins do not lose the protein activity.
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