The AMPK kinase assay kit can be used to measure the activity of AMPK for 96 assays. Applications of the kit include screening activators or inhibitors of AMPK, evaluating the effects of pharmacological agents on AMPK in vitro or monitoring the effects of purification of AMPK. MBL International's AMPK kinase assay kit is highly cited. Bulk pricing available.

Product Specifications
Application ELISA


Documents & Links for CycLex® AMPK Kinase Assay Kit
Datasheet CycLex® AMPK Kinase Assay Kit Datasheet

Documents & Links for CycLex® AMPK Kinase Assay Kit
Datasheet CycLex® AMPK Kinase Assay Kit Datasheet



Citations for CycLex® AMPK Kinase Assay Kit – 51 Found
Wang, Ying; Nishi, Masahiro; Doi, Asako; Shono, Takeshi; Furukawa, Yasushi; Shimada, Takeshi; Furuta, Hiroto; Sasaki, Hideyuki; Nanjo, Kishio. Ghrelin inhibits insulin secretion through the AMPK-UCP2 pathway in beta cells. Febs Letters. 2010;584(8):1503-8.  PubMed
Li, Dan; Wang, Duan; Wang, Yun; Ling, Wenhua; Feng, Xiang; Xia, Min. Adenosine monophosphate-activated protein kinase induces cholesterol efflux from macrophage-derived foam cells and alleviates atherosclerosis in apolipoprotein E-deficient mice. The Journal Of Biological Chemistry. 2010;285(43):33499-33509.  PubMed
Vingtdeux, Valérie; Chandakkar, Pallavi; Zhao, Haitian; d'Abramo, Cristina; Davies, Peter; Marambaud, Philippe. Novel synthetic small-molecule activators of AMPK as enhancers of autophagy and amyloid-β peptide degradation. Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology. 2011;25(1):219-31.  PubMed
Tang, Yun-Chi; Williams, Bret R; Siegel, Jake J; Amon, Angelika. Identification of aneuploidy-selective antiproliferation compounds. Cell. 2011;144(4):499-512.  PubMed
Yeh, Chi-Hsiao; Chen, Tzu-Ping; Wang, Yao-Chang; Lin, Yu-Min; Fang, Shu-Wen. AMP-activated protein kinase activation during cardioplegia-induced hypoxia/reoxygenation injury attenuates cardiomyocytic apoptosis via reduction of endoplasmic reticulum stress. Mediators Of Inflammation. 2010:130636.  PubMed
Wang, Zhigang; Pini, Maria; Yao, Tong; Zhou, Zhanxiang; Sun, Changhao; Fantuzzi, Giamila; Song, Zhenyuan. Homocysteine suppresses lipolysis in adipocytes by activating the AMPK pathway. American Journal Of Physiology. Endocrinology And Metabolism. 2011;301(4):E703-12.  PubMed
Yokoyama, Yuichi; Iguchi, Kazuhiro; Usui, Shigeyuki; Hirano, Kazuyuki. AMP-activated protein kinase modulates the gene expression of aquaporin 9 via forkhead box a2. Archives Of Biochemistry And Biophysics. 2011;515(1-2):80-8.  PubMed
Lee, Mak-Soon; Kim, In-Hwan; Kim, Chong-Tai; Kim, Yangha. Reduction of body weight by dietary garlic is associated with an increase in uncoupling protein mRNA expression and activation of AMP-activated protein kinase in diet-induced obese mice. The Journal Of Nutrition. 2011;141(11):1947-53.  PubMed
Greco, Steven J; Hamzelou, Ashkan; Johnston, Jane M; Smith, Mark A; Ashford, J Wesson; Tezapsidis, Nikolaos. Leptin boosts cellular metabolism by activating AMPK and the sirtuins to reduce tau phosphorylation and β-amyloid in neurons. Biochemical And Biophysical Research Communications. 2011;414(1):170-4.  PubMed
Miyokawa-Gorin, Kaoru; Takahashi, Kazuto; Handa, Keiko; Kitahara, Atsuko; Sumitani, Yoshikazu; Katsuta, Hidenori; Tanaka, Toshiaki; Nishida, Susumu; Yoshimoto, Katsuhiko; Ohno, Hideki; Ishida, Hitoshi. Induction of mitochondrial uncoupling enhances VEGF₁₂₀ but reduces MCP-1 release in mature 3T3-L1 adipocytes: possible regulatory mechanism through endogenous ER stress and AMPK-related pathways. Biochemical And Biophysical Research Communications. 2012;419(2):200-5.  PubMed
Magnoni, Leonardo J; Vraskou, Yoryia; Palstra, Arjan P; Planas, Josep V. AMP-activated protein kinase plays an important evolutionary conserved role in the regulation of glucose metabolism in fish skeletal muscle cells. Plos One. 7(2):e31219.  PubMed
Bruckbauer, Antje; Zemel, Michael B. Synergistic effects of metformin, resveratrol, and hydroxymethylbutyrate on insulin sensitivity. Diabetes, Metabolic Syndrome And Obesity : Targets And Therapy. 6:93-102.  PubMed
Figarola, James L; Rahbar, Samuel. Small‑molecule COH-SR4 inhibits adipocyte differentiation via AMPK activation. International Journal Of Molecular Medicine. 2013;31(5):1166-76.  PubMed
Yamada, Shigeru; Kotake, Yaichiro; Sekino, Yuko; Kanda, Yasunari. AMP-activated protein kinase-mediated glucose transport as a novel target of tributyltin in human embryonic carcinoma cells. Metallomics : Integrated Biometal Science. 2013;5(5):484-91.  PubMed
Takikawa, Masathito; Kumagai, Aya; Hirata, Harumi; Soga, Minoru; Yamashita, Yoko; Ueda, Manabu; Ashida, Hitoshi; Tsuda, Takanori. 10-Hydroxy-2-decenoic acid, a unique medium-chain fatty acid, activates 5'-AMP-activated protein kinase in L6 myotubes and mice. Molecular Nutrition & Food Research. 2013;57(10):1794-802.  PubMed
Seo, Jun-Young; Cresswell, Peter. Viperin regulates cellular lipid metabolism during human cytomegalovirus infection. Plos Pathogens. 9(8):e1003497.  PubMed
Zhang, Ximei; Wang, Zhigang; Li, Jiaxin; Gu, Dongfang; Li, Songtao; Shen, Chen; Song, Zhenyuan. Increased 4-hydroxynonenal formation contributes to obesity-related lipolytic activation in adipocytes. Plos One. 8(8):e70663.  PubMed
Hsu, Chin-Yuan; Chuang, Yu-Lung. Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera). The Journals Of Gerontology. Series A, Biological Sciences And Medical Sciences. 2014;69(8):955-64.  PubMed
Liang, Yan; Huang, Bosheng; Song, Erfei; Bai, Bo; Wang, Yu. Constitutive activation of AMPK α1 in vascular endothelium promotes high-fat diet-induced fatty liver injury: role of COX-2 induction. British Journal Of Pharmacology. 2014;171(2):498-508.  PubMed
Kim, Joo-Yeon; Lee, Mak-Soon; Jung, Sunyoon; Joo, Hyunjin; Kim, Chong-Tai; Kim, In-Hwan; Seo, Sangjin; Oh, Soojung; Kim, Yangha. Anti-obesity efficacy of nanoemulsion oleoresin capsicum in obese rats fed a high-fat diet. International Journal Of Nanomedicine. 9:301-10.  PubMed
Zadra, Giorgia; Photopoulos, Cornelia; Tyekucheva, Svitlana; Heidari, Pedram; Weng, Qing Ping; Fedele, Giuseppe; Liu, Hong; Scaglia, Natalia; Priolo, Carmen; Sicinska, Ewa; Mahmood, Umar; Signoretti, Sabina; Birnberg, Neal; Loda, Massimo. A novel direct activator of AMPK inhibits prostate cancer growth by blocking lipogenesis. Embo Molecular Medicine. 2014;6(4):519-38.  PubMed
Magnoni, Leonardo J; Palstra, Arjan P; Planas, Josep V. Fueling the engine: induction of AMP-activated protein kinase in trout skeletal muscle by swimming. The Journal Of Experimental Biology. 2014;217(Pt 10):1649-52.  PubMed
Bruckbauer, Antje; Zemel, Michael B. Synergistic effects of polyphenols and methylxanthines with Leucine on AMPK/Sirtuin-mediated metabolism in muscle cells and adipocytes. Plos One. 9(2):e89166.  PubMed
Park, Hwangseo; Eom, Jae-Won; Kim, Yang-Hee. Consensus scoring approach to identify the inhibitors of AMP-activated protein kinase α2 with virtual screening. Journal Of Chemical Information And Modeling. 2014;54(7):2139-46.  PubMed
Hsu, Chin-Yuan; Hu, Ting-Hui. Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees. Biogerontology. 2014;15(4):389-400.  PubMed
Kobashigawa, Laura C; Xu, Yan Chun; Padbury, James F; Tseng, Yi-Tang; Yano, Naohiro. Metformin protects cardiomyocyte from doxorubicin induced cytotoxicity through an AMP-activated protein kinase dependent signaling pathway: an in vitro study. Plos One. 9(8):e104888.  PubMed
Lee, Mak-Soon; Jung, Sunyoon; Oh, Soojung; Shin, Yoonjin; Kim, Chong-Tai; Kim, In-Hwan; Kim, Yangha. Effect of high hydrostatic pressure extract of fresh ginseng on adipogenesis in 3T3-L1 adipocytes. Journal Of The Science Of Food And Agriculture. 2015;95(12):2409-15.  PubMed
Noda, Kazuki; Nakajima, Sota; Godo, Shigeo; Saito, Hiroki; Ikeda, Shohei; Shimizu, Toru; Enkhjargal, Budbazar; Fukumoto, Yoshihiro; Tsukita, Sohei; Yamada, Tetsuya; Katagiri, Hideki; Shimokawa, Hiroaki. Rho-kinase inhibition ameliorates metabolic disorders through activation of AMPK pathway in mice. Plos One. 9(11):e110446.  PubMed
Soga, Minoru; Ohashi, Ayaka; Taniguchi, Megumi; Matsui, Toshiro; Tsuda, Takanori. The di-peptide Trp-His activates AMP-activated protein kinase and enhances glucose uptake independently of insulin in L6 myotubes. Febs Open Bio. 4:898-904.  PubMed
Sato, Shuichi; Ogura, Yuji; Tajrishi, Marjan M; Kumar, Ashok. Elevated levels of TWEAK in skeletal muscle promote visceral obesity, insulin resistance, and metabolic dysfunction. Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology. 2015;29(3):988-1002.  PubMed
Zhou, Xihong; Wu, Weiche; Chen, Jingqing; Wang, Xinxia; Wang, Yizhen. AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid. Nutrition & Metabolism. 12:10.  PubMed
Akhtar, Nosheen; Syed, Deeba N; Khan, Mohammad Imran; Adhami, Vaqar M; Mirza, Bushra; Mukhtar, Hasan. The pentacyclic triterpenoid, plectranthoic acid, a novel activator of AMPK induces apoptotic death in prostate cancer cells. Oncotarget. 2016;7(4):3819-31.  PubMed
Zhao, Changfu; Zhang, Qiao; Yu, Tao; Sun, Shudong; Wang, Wenjun; Liu, Guangyao. Hypoxia promotes drug resistance in osteosarcoma cells via activating AMP-activated protein kinase (AMPK) signaling. Journal Of Bone Oncology. 2016;5(1):22-9.  PubMed
Niopek, Katharina; Üstünel, Bilgen Ekim; Seitz, Susanne; Sakurai, Minako; Zota, Annika; Mattijssen, Frits; Wang, Xiaoyue; Sijmonsma, Tjeerd; Feuchter, Yvonne; Gail, Anna M; Leuchs, Barbara; Niopek, Dominik; Staufer, Oskar; Brune, Maik; Sticht, Carsten; Gretz, Norbert; Müller-Decker, Karin; Hammes, Hans-Peter; Nawroth, Peter; Fleming, Thomas; Conkright, Michael D; Blüher, Matthias; Zeigerer, Anja; Herzig, Stephan; Berriel Diaz, Mauricio. A Hepatic GAbp-AMPK Axis Links Inflammatory Signaling to Systemic Vascular Damage. Cell Reports. 2017;20(6):1422-1434.  PubMed
Jang, Jaewoong; Jung, Yoonju; Seo, Seong Jun; Kim, Seok-Min; Shim, Yae Jie; Cho, Soo Hyun; Chung, Sang-In; Yoon, Yoosik. Berberine activates AMPK to suppress proteolytic processing, nuclear translocation and target DNA binding of SREBP-1c in 3T3-L1 adipocytes. Molecular Medicine Reports. 2017;15(6):4139-4147.  PubMed
Chen, Xi; Xie, Chun; Fan, Xing-Xing; Jiang, Ze-Bo; Wong, Vincent Kam-Wai; Xu, Jia-Hui; Yao, Xiao-Jun; Liu, Liang; Leung, Elaine Lai-Han. Novel direct AMPK activator suppresses non-small cell lung cancer through inhibition of lipid metabolism. Oncotarget. 2017;8(56):96089-96102.  PubMed
Rovira, Mireia; Arrey, Gerard; Planas, Josep V. Exercise-Induced Hypertrophic and Oxidative Signaling Pathways and Myokine Expression in Fast Muscle of Adult Zebrafish. Frontiers In Physiology. 8:1063.  PubMed
Wang, Hongyun; Shen, Xiyue; Tian, Guoxiong; Shi, Xili; Huang, Wei; Wu, Yongguang; Sun, Lei; Peng, Can; Liu, Shasha; Huang, Ying; Chen, Xiaoyu; Zhang, Fang; Chen, Yingjie; Ding, Wenjun; Lu, Zhongbing. AMPKα2 deficiency exacerbates long-term PM(2.5) exposure-induced lung injury and cardiac dysfunction. Free Radical Biology & Medicine. 2018;121:202-214.  PubMed
Kang, Myung-Ji; Kwon, Eun-Bin; Ryu, Hyung Won; Lee, Seoghyun; Lee, Jae-Won; Kim, Doo-Young; Lee, Mi Kyeong; Oh, Sei-Ryang; Lee, Hyun-Sun; Lee, Su Ui; Kim, Mun-Ock. Polyacetylene From Dendropanax morbifera Alleviates Diet-Induced Obesity and Hepatic Steatosis by Activating AMPK Signaling Pathway. Frontiers In Pharmacology. 9:537.  PubMed
Mok, Simon W F; Zeng, Wu; Niu, Yuzhen; Coghi, Paolo; Wu, Yujun; Sin, Wai Man; Ng, Sio Ian; Gordillo-Martínez, Flora; Gao, Jia Yin; Law, Betty Y K; Liu, Liang; Yao, Xiaojun; Wong, Vincent K W. A Method for Rapid Screening of Anilide-Containing AMPK Modulators Based on Computational Docking and Biological Validation. Frontiers In Pharmacology. 9:710.  PubMed
Kawasumi, Koh; Murai, Tae; Mizorogi, Takayuki; Okada, Yuki; Yamamoto, Ichiro; Suruga, Kohei; Kadokura, Kazunari; Arai, Toshiro. Changes in Plasma Metabolites Concentrations in Obese Dogs Supplemented With Anti-oxidant Compound. Frontiers In Nutrition. 5:74.  PubMed
Lee, Mak-Soon; Kim, Yangha. Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation. Molecules (Basel, Switzerland). 2018;23(8)  PubMed
Dai, Sheng-Nan; Hou, Ai-Jie; Zhao, Shu-Mei; Chen, Xiao-Ming; Huang, Hua-Ting; Chen, Bo-Han; Kong, Hong-Liang. Ginsenoside Rb1 Ameliorates Autophagy of Hypoxia Cardiomyocytes from Neonatal Rats via AMP-Activated Protein Kinase Pathway. Chinese Journal Of Integrative Medicine. 2019;25(7):521-528.  PubMed
Kim, Seunghae; Lee, Mak-Soon; Jung, Sunyoon; Son, Hye-Yeon; Park, Seonyoung; Kang, Bori; Kim, Seog-Young; Kim, In-Hwan; Kim, Chong-Tai; Kim, Yangha. Ginger Extract Ameliorates Obesity and Inflammation via Regulating MicroRNA-21/132 Expression and AMPK Activation in White Adipose Tissue. Nutrients. 2018;10(11)  PubMed
Kim, Seog-Young; Lee, Mak-Soon; Chang, Eugene; Jung, Sunyoon; Ko, Hyunmi; Lee, Eunyoung; Lee, Soojin; Kim, Chong-Tai; Kim, In-Hwan; Kim, Yangha. Tartary Buckwheat Extract Attenuated the Obesity-Induced Inflammation and Increased Muscle PGC-1a/SIRT1 Expression in High Fat Diet-Induced Obese Rats. Nutrients. 2019;11(3)  PubMed
Chang, Eugene; Kim, Yangha. Vitamin D Ameliorates Fat Accumulation with AMPK/SIRT1 Activity in C2C12 Skeletal Muscle Cells. Nutrients. 2019;11(11)  PubMed
Si, Yuan; Wang, Jiu; Liu, Xuewen; Zhou, Tong; Xiang, Yuchen; Zhang, Te; Wang, Xianhui; Feng, Tingting; Xu, Li; Yu, Qingqing; Zhao, Huzi; Liu, Ying. Ethoxysanguinarine, a Novel Direct Activator of AMP-Activated Protein Kinase, Induces Autophagy and Exhibits Therapeutic Potential in Breast Cancer Cells. Frontiers In Pharmacology. 10:1503.  PubMed
Wu, Yezi; Si, Yuan; Xiang, Yuchen; Zhou, Tong; Liu, Xuewen; Wu, Mingwei; Li, Wenjuan; Zhang, Te; Xiang, Ke; Zhang, Liang; Zhao, Huzi; Liu, Ying. Polyphyllin I activates AMPK to suppress the growth of non-small-cell lung cancer via induction of autophagy. Archives Of Biochemistry And Biophysics. 2020;687:108285.  PubMed
Lee, Mak-Soon; Kim, Yangha. Mulberry Fruit Extract Ameliorates Adipogenesis via Increasing AMPK Activity and Downregulating MicroRNA-21/143 in 3T3-L1 Adipocytes. Journal Of Medicinal Food. 2020;23(3):266-272.  PubMed
Ren, Gang; Guo, Jiang-Hong; Qian, Yu-Zhen; Kong, Wei-Jia; Jiang, Jian-Dong. Berberine Improves Glucose and Lipid Metabolism in HepG2 Cells Through AMPKα1 Activation. Frontiers In Pharmacology. 11:647.  PubMed
Kondo, Koyuki; Mori, Masaru; Tomita, Masaru; Arakawa, Kazuharu. Pre-treatment with D942, a furancarboxylic acid derivative, increases desiccation tolerance in an anhydrobiotic tardigrade Hypsibius exemplaris. Febs Open Bio. 2020;10(9):1774-1781.  PubMed