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For cryopreservation of hematopoieticstem cells, dimethyl sulfoxide (DMSO) has generally been used as a cryoprotectant.
In the case ofcryopreservation with DMSO alone, it was common to be stored in liquid nitrogen (-196°C) after frozen by the stepwisefreezing method with a program freezer. However, from the early 1980's, simple cell freezing methods that do not use a program freezer have been studiedusing DMSO and hydroxyethyl starch (HES) for hematopoietic stem cells in bone marrow.
In Japan, studies on application of simple cell freezing method using DMSO and HES toperipheral blood stem cells have beenadvanced, and the optimum concentrations of HES and DMSO in the simple cell freezing method were decided 6% HES, 5% DMSO. This simple cell freezing method enabled stable cryopreservation for a short period of time (6 months to 1 year and a half) using a -80°C freezer without stepwise freezing method.
Features
- Confirmed eligibility for product materials such as regenerative medicine * 1
- Registered drug substance, etc. Registered (MF)
- Manufactured at our GMP-compatible facility in Japan
- Non-clinical safety test completed
- Uses USP grade DMSO
- Free of animal-derived ingredients
- Disclosure of composition
- Perform sterility test, endotoxin, mycoplasma denial test
- Final DMSO concentration is 5%
Composition information
- Hydroxyethyl starch
- Dimethyl sulfoxide
- Saline
Examples of cells that can be frozen with CP-1 ® High Grade
| Adhesive cells | |||
|---|---|---|---|
| Human iPS cells | PFX # 9 | Human ES cells | H9 |
| Human adipose tissue-derived mesenchymal stem cells | MSC-AT | Human bone marrow-derived mesenchymal stem cells | MSC-BM |
| Human neonatal-derived skin fibroblasts | NHDF-Neo | Human fetal kidney-derived cells | HEK293 |
| Chinese hamster ovary cells | CHO-K1 | Human osteoblasts | Human Osteobrast |
| Floating cells | |
|---|---|
| Mouse hybridoma | SJK-287-38 |
| Human hematopoietic stem cells (human peripheral blood stem cells, human cord blood stem cells, human bone marrow stem cells) |
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| Documents & Links for CP-1(R) High Grade | |
| Manual | CP-1(R) High Grade Manual |
| Documents & Links for CP-1(R) High Grade | |
| Manual | CP-1(R) High Grade Manual |