CP-1® High Grade Cryoprotectant Reagent for Hematopoietic Stem Cells

Kyokuto Pharmaceutical Indust

Catalog No.: KYO-27207

For cryopreservation of hematopoietic stem cells, dimethyl sulfoxide (DMSO) has generally been used as a cryoprotectant.

In the case of cryopreservation with DMSO alone, it was common to store hematopoietic stem cells in liquid nitrogen (-196°C) after being frozen by the stepwise freezing method with a program freezer. However, from the early 1980's, simple cell freezing methods that do not use a program freezer have been studied using DMSO and hydroxyethyl starch (HES) for hematopoietic stem cells in bone marrow.

In Japan, studies on the application of a simple cell freezing method using DMSO and HES to peripheral blood stem cells have been conducted, and the optimum concentrations of HES and DMSO in the simple cell freezing method were determined at 6% HES and 5% DMSO. This simple cell freezing method enabled stable cryopreservation for a short period of time (6 months to 1 year and a half) using a -80°C freezer without using the stepwise freezing method.

Features

• Confirmed eligibility for product materials such as regenerative medicine
• Registered drug substance, etc. Registered (MF)
• Manufactured at our GMP-compatible facility in Japan
• Non-clinical safety test completed
• Uses USP grade DMSO
• Free of animal-derived ingredients
• Disclosure of composition
• Perform sterility test, endotoxin, mycoplasma denial test
• Final DMSO concentration is 5%

Composition information

• Hydroxyethyl starch
• Dimethyl sulfoxide
• Saline

Examples of cells that can be frozen with CP-1^® High Grade

Adherent Cells
Human iPS cells	PFX # 9	Human ES cells	H9
Human adipose tissue-derived mesenchymal stem cells	MSC-AT	Human bone marrow-derived mesenchymal stem cells	MSC-BM
Human neonatal-derived skin fibroblasts	NHDF-Neo	Human fetal kidney-derived cells	HEK293
Chinese hamster ovary cells	CHO-K1	Human osteoblasts	Human Osteobrast

Suspension Cells
Mouse hybridoma	SJK-287-38
Human hematopoietic stem cells (human peripheral blood stem cells, human cord blood stem cells, human bone marrow stem cells)