COS banker II [Cell Freezing Medium]

Cosmo Bio

Catalog No.: KOJ-COS-CFM02

Product Description

COS Banker II is a one-step cell-freezing medium developed for the long-term preservation of human lymphocytes and other hematopoietic cells. Suspend cultured cells in COS Banker II and store at −80 °C; liquid-nitrogen storage is not required. The medium contains 10% DMSO and GMP-grade human serum albumin as a stabilizer.

Feature and Advantages

• Ready to use with no dilution required
• Supports direct freezing and long-term storage at −80 °C
• Supports high cell viability after thawing
• Contains GMP-grade human serum albumin as a stabilizer
• Contains no whole serum
• Tested for endotoxin and bacterial contamination

Components

• COS Banker II, 120 mL
• Contains 10%  DMSO
• Contains human serum albumin
• Contains no whole serum

Store at 2–8 °C in a dark place.
Expiration date: 2 years from date of manufacture

Specifications

Product name	COS banker	COS banker II
Product Code	KOJ-COS-CFM01	KOJ-COS-CFM02
Size	120 mL	120 mL
Specification	Contains DMSO No serum	Contains DMSO Contains Human Serum Albumin No serum
Storage	Store at 2-8°C in a dark place	Store at 2-8°C in a dark place
Expiry Date	2 years from date of manufacture	2 years from date of manufacture
Application Tested Cell	iPS cells (Dotcom), Daudi, HeLa S3, K562, Raji, MOLT-4, CTLL-2, NIH/3T3, PC-12, CHO-K1, Vero cells, MDCK cells, MCF-7, HepG2, P3U1, Fibroblasts, Epidermal keratinocytes, Vascular endothelial cells, Hybridomas, SF9 (insect cells)	Activated lymphocytes derived from human peripheral blood, Daudi, K562, Raji, K652, Molt-4F, CTLL-2, MCF-7, HepG2, P3U1,Fibroblasts, Epidermal keratinocytes, Vascular endothelial cells, Hybridomas, Vero cells, MDCK cells, HeLa, SF9 (insect cells)

Applications tested

• Activated lymphocytes derived from human peripheral blood

COS Banker II may also be used with cells compatible with COS Banker.

Instructions

1. Collect cells during the logarithmic growth phase. Place approximately 5 × 10⁵ to 1 × 10⁷ cells in a cryovial and centrifuge for five minutes at 1,200 rpm.
2. Add 1 mL of COS Banker II to the cell pellet and resuspend thoroughly. Freeze and store at −80 °C.
3. If liquid-nitrogen storage is desired, freeze the cells at −80 °C for at least one day before transferring them to liquid nitrogen.
4. Thaw rapidly in a 37 °C water bath.

For optimal post-thaw viability, freeze healthy cells during the logarithmic growth phase.
For research use only. Not for diagnostic or clinical use.