CD147/CD9 Exosome ELISA Kit

Hakarel

Catalog No.: HAK-HEL14709-1

Background and Measurement Principal

Cluster of differentiation (CD) 147 is a transmembrane glycoprotein that is highly expressed 
at the tumor cell surface, which stimulates fibroblasts to produce a large number of matrix 
metalloproteinases and promotes tumor invasion and metastasis and tumor-induced 
angiogenesis.１）
It has been suggested that an increase in CD147-positive exosomes released into the 
bloodstream from cancer cells may be useful as a diagnostic and prognostic biomarker for 
colorectal cancer and other diseases. 2）
This product is a two-step sandwich ELISA kit that uses high-performance antibodies against 
CD9 and CD147, which are exosome markers, to detect CD147 molecules expressed on the 
surface of exosomes secreted by cells in human serum or cell culture medium.

Features

・Directly quantitate CD147-positive exosomes in human blood samples or cell culture 
supernatant.
・No special equipment is required. Standard microplate reader capable of reading at 450nm
 will do the job.
・Utilize CD147/CD9 fusion protein (Standard Protein), instead of unstable/hard to store 
exosome itself, to implement stability and reproducibility.
・Normalization with a standard curve using CD147/CD9 fusion protein (Standard Protein) 
 enable to relative quantitate each sample.
・Detect human CD147-positive exosomes by two-step sandwich method using immobilized 
 anti-CD147 antibody and HRP conjugated anti-CD9 antibody.