Cluster of differentiation (CD) 147 is a transmembrane glycoprotein that is highly expressed at the tumor cell surface, which stimulates fibroblasts to produce a large number of matrix metalloproteinases and promotes tumor invasion and metastasis and tumor-induced angiogenesis.１） It has been suggested that an increase in CD147-positive exosomes released into the bloodstream from cancer cells may be useful as a diagnostic and prognostic biomarker for colorectal cancer and other diseases. 2） This product is a two-step sandwich ELISA kit that uses high-performance antibodies against CD9 and CD147, which are exosome markers, to detect CD147 molecules expressed on the surface of exosomes secreted by cells in human serum or cell culture medium.
Features
・Directly quantitate CD147-positive exosomes in human blood samples or cell culture supernatant. ・No special equipment is required. Standard microplate reader capable of reading at 450nm will do the job. ・Utilize CD147/CD9 fusion protein (Standard Protein), instead of unstable/hard to store exosome itself, to implement stability and reproducibility. ・Normalization with a standard curve using CD147/CD9 fusion protein (Standard Protein) enable to relative quantitate each sample. ・Detect human CD147-positive exosomes by two-step sandwich method using immobilized anti-CD147 antibody and HRP conjugated anti-CD9 antibody.
