Bone Resorption Assay Kit

Cosmo Bio

Catalog No.: CSR-BRA-24KIT

Background

Useful for the research of skeletal metabolism

This product is an assay kit for the measurement of bone resorption activity using a fluoresceinated calcium phosphate-coated plate (patent pending). The coated calcium phosphate is first bound to fluoresceinamine-labeled chondroitin sulfate (FACS), which is released from the calcium phosphate layer into conditioned medium by osteoclastic resorption activity. Bone resorption activity is evaluated by simply measuring the fluorescence intensity of the conditioned medium. This assayprovides a rapid evaluation system unlike that of the traditional pit assay

Features

1. Bone resorption activity is evaluated simply by measuring the fluorescence intensity of the medium.
2. The fluorescence excitation and emission wavelengthsare identical to those for the commonly-used fluorescent dye, FITC.
3. Microscopic observation of cell morphology is possible.
4. Pit area analysis is also possible after removing the cells.
5. Ready to use, sterile components.

References (CSR-BRA-24P, CSR-BRA-48P)

1) Kim JL, Osteogenic activity of yellow flag iris (Iris pseudacorus) extract modulating differentiation of osteoblasts and osteoclasts. Am J Chin Med. 2012, 40(6):1289-305.
2) Wu K, Dextromethorphan inhibits osteoclast differentiation by suppressing RANKLinduced nuclear factor-κB activation. Osteoporos Int. 2013, 24(8):2201-14.
3) Kanzaki H, The keap1/nrf2 protein axis plays a role in osteoclast differentiation by regulating intracellular reactive oxygen species signaling. J Biol Chem. 2013, 288(32):23009-20.
4) Moon SJ, Temporal differential effects of proinflammatory cytokines on osteoclastogenesis. Int J Mol Med. 2013, 31(4):769-77. 5) Fabre C, Dual inhibition of canonical and noncanonical NF-κB pathways demonstrates significant antitumor activities in multiple myeloma. Clin Cancer Res. 2012, 18(17):4669-81.
6) Harada K, Polyphosphate-Mediated Inhibition of Tartrate-Resistant Acid Phosphatase and Suppression of Bone Resorption of Osteoclasts. PLoS One. 2013, 8(11): e78612 7) Hokugo A, Equilibrium-dependent bisphosphonate interaction with crystalline bone mineral explains anti-resorptive pharmacokinetics and prevalence of osteonecrosis of the jaw in rats. Bone. 2013, 53:59-68.
8) Eda H, A novel Bruton's tyrosine kinase inhibitor CC-292 in combination with the proteasome inhibitor carfilzomib impacts the bone microenvironment in a multiple myeloma model with resultant antimyeloma activity. Leukemia. 2014, 28(9):1892- 901 9) Orosa B, Lysophosphatidic acid receptor inhibition as a new multipronged treatment for rheumatoid arthritis. Ann Rheum Dis. 2014, 73(1):298-305 10) Sharma R, Caspase-2 maintains bone homeostasis by inducing apoptosis of oxidatively-damaged osteoclasts. PLoS One. 2014, 9(4):e93696 11) Hsieh CJ, Wedelolactone inhibits breast cancer-induced osteoclastogenesis by decreasing Akt/mTOR signaling. Int J Oncol, 2015, 46(2):555-62. 12) Mishima K, Lansoprazole Upregulates Polyubiquitination of the TNF ReceptorAssociated Factor 6 and Facilitates Runx2-mediated Osteoblastogenesis. EBioMedicine. 2015, 2(12):2046-61
13) Ishikawa S, Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells. Evid Based Complement Alternat Med. 2016;2016:7262054 14) Wijekoon S, Chronological differential effects of pro-inflammatory cytokines on RANKL-induced osteoclast differentiation of canine bone marrow-derived macrophages. J Vet Med Sci. 2017, 79(12):2030-2035.
15) Kanzaki H, Nuclear Nrf2 induction by protein transduction attenuates osteoclastogenesis. Free Radic Biol Med. 2014, 77:239-48.
16) Maruyama K, Nociceptors Boost the Resolution of Fungal Osteoinflammation via the TRP Channel-CGRP-Jdp2 Axis. Cell Rep, 2017, 19(13):2730-2742.
17) Wijekoon HMS, Inhibitory effects of sodium pentosan polysulfate on formation and function of osteoclasts derived from canine bone marrow. BMC Vet Res. 2018, 14(1):152. 18) Jin SH, Actin-binding LIM Protein 1 Regulates Receptor Activator of NF-κB LigandMediated Osteoclast Differentiation and Motility. BMB Rep. 2018 Jul;51(7):356-361. 19) Nishida M, S100A12 facilitates osteoclast differentiation from human monocytes. PLoS One. 2018, 13(9):e0204140.
20) Ohnuma K, MicroRNA-124 inhibits TNF-α- and IL-6-induced osteoclastogenesis. Rheumatol Int. 2019, 39(4):689-695.
21) Kumagai M, Fluorinated Kavalactone Inhibited RANKL-Induced Osteoclast Differentiation of RAW264 Cells. Biol Pharm Bull. 2020, 43(5):898-903. 22) Lee EJ, Coumarin Ameliorates Impaired Bone Turnover by Inhibiting the Formation of Advanced Glycation End Products in Diabetic Osteoblasts and Osteoclasts. Biomolecules, 2020, 10(7):1052.
23) Nishioku T, (-)-Epigallocatechin-3-gallate inhibits RANKL-induced osteoclastogenesis via downregulation of NFATc1 and suppression of HO-1-HMGB1- RAGE pathway. Biomed Res. 2020, 41(6):269-277.
24) Park KH, Inhibitory Effect of Rosae Multiflorae Fructus Extracts on the Receptor Activator of NF-κB Ligand-Induced Osteoclastogenesis through Modulation of P38- and Ca 2+-Mediated Nuclear Factor of Activated T-Cells Cytoplasmic 1 Expression. J Bone Metab. 2020, 27(1):53-63.
25) Wada S, Bach1 Inhibition Suppresses Osteoclastogenesis via Reduction of the Signaling via Reactive Oxygen Species by Reinforced Antioxidation. Front Cell Dev Biol, 2020, 8:740.
26) Jeong S, Isoliquiritigenin Derivatives Inhibit RANKL-Induced Osteoclastogenesis by Regulating p38 and NF-κB Activation in RAW 264.7 Cells. Molecules, 2020, 25(17):3908. 27) Lee EJ, Hydroxycoumarin Scopoletin Inhibits Bone Loss through Enhancing Induction of Bone Turnover Markers in a Mouse Model of Type 2 Diabetes. Biomedicines, 2021, 9(6):648. 28) Zhen G, An antibody against Siglec-15 promotes bone formation and fracture healing by increasing TRAP + mononuclear cells and PDGF-BB secretion. Bone Res, 2021, 9(1):47.
29) Cho E, Identification of Novel Genes for Cell Fusion during Osteoclast Formation. Int J Mol Sci, 2022, 23(12):6421. 30) Kelk P, Aggregatibacter actinomycetemcomitans Leukotoxin Activates the NLRP3 Inflammasome and Cell-to-Cell Communication. Pathogens, 2022,11(2):159.
31) Wijekoon S, Pentosan polysulfate regulates hepcidin 1-facilitated formation and function of osteoclast derived from canine bone marrow. PLoS One, 2022, 17(3):e0265596. 32) Okawa H, Mechanism of bisphosphonate-related osteonecrosis of the jaw (BRONJ) revealed by targeted removal of legacy bisphosphonate from jawbone using competing inert hydroxymethylene diphosphonate. eLife. 2022; 11: e76207.
33) Chen Qi, Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease. Front Endocrinol (Lausanne). 2022; 13: 913979. 34) Kang IH, Quantitative increase in T regulatory cells enhances bone remodeling in osteogenesis imperfecta. iScience. 2022, 25(9):104818.

Citations for Bone Resorption Assay Kit – 63 Found

Cheng, Tsung-Lin; Lai, Chao-Han; Shieh, Shyh-Jou; Jou, Yin-Bo; Yeh, Jwu-Lai; Yang, Ai-Lun; Wang, Yan-Hsiung; Wang, Chau-Zen; Chen, Chung-Hwan; Shi, Guey-Yueh; Ho, Mei-Ling; Wu, Hua-Lin. Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss. Scientific Reports. 2016;6( 27311356):28340. 

Park, Sil; Kanayama, Keiichi; Kaur, Kawaljit; Tseng, Han-Ching Helen; Banankhah, Sina; Quje, Davood Talebi; Sayre, James W; Jewett, Anahid; Nishimura, Ichiro. Osteonecrosis of the Jaw Developed in Mice: DISEASE VARIANTS REGULATED BY γδ T CELLS IN ORAL MUCOSAL BARRIER IMMUNITY. The Journal Of Biological Chemistry. 2015;290(28):17349-66. 

Kang, J H; Ko, H M; Moon, J S; Yoo, H I; Jung, J Y; Kim, M S; Koh, J T; Kim, W J; Kim, S H. Osteoprotegerin expressed by osteoclasts: an autoregulator of osteoclastogenesis. Journal Of Dental Research. 2014;93(11):1116-23. 

Kim, Yun-Ho; Kim, Jung-Lye; Lee, Eun-Jung; Park, Sin-Hye; Han, Seon-Young; Kang, Soon Ah; Kang, Young-Hee. Fisetin antagonizes cell fusion, cytoskeletal organization and bone resorption in RANKL-differentiated murine macrophages. The Journal Of Nutritional Biochemistry. 2014;25(3):295-303. 

Lee, Chang Won; Seo, Jeong Yeon; Choi, Ji Won; Lee, Jisun; Park, Joo Woong; Lee, Jae Yeon; Hwang, Kyo-Yeol; Park, Young Shik; Park, Yong Il. Potential anti-osteoporotic activity of low-molecular weight hyaluronan by attenuation of osteoclast cell differentiation and function in vitro. Biochemical And Biophysical Research Communications. 2014;449(4):438-43. 

Kuo, Chih-Hsin; Chen, Kuei-Fang; Chou, Shah-Hwa; Huang, Ya-Fang; Wu, Cheng-Ying; Cheng, Da-En; Chen, Yu-Wen; Yang, Chih-Jen; Hung, Jen-Yu; Huang, Ming-Shyan. Lung tumor-associated dendritic cell-derived resistin promoted cancer progression by increasing Wolf-Hirschhorn syndrome candidate 1/Twist pathway. Carcinogenesis. 2013;34(11):2600-9. 

Hsu, Li-Chien; Reddy, Sakamuri V; Yilmaz, Özlem; Yu, Hong. Sphingosine-1-Phosphate Receptor 2 Controls Podosome Components Induced by RANKL Affecting Osteoclastogenesis and Bone Resorption. Cells. 2019;8(1) 

Ma, Yong; Wang, Lining; Zheng, Suyang; Xu, Jiake; Pan, Yalan; Tu, Pengcheng; Sun, Jie; Guo, Yang. Osthole inhibits osteoclasts formation and bone resorption by regulating NF-κB signaling and NFATc1 activations stimulated by RANKL. Journal Of Cellular Biochemistry. 2019;120(9):16052-16061. 

Lu, Jiajia; Shi, Xiaojian; Fu, Qiang; Han, Yaguang; Zhu, Lei; Zhou, Zhibin; Li, Yongchuan; Lu, Nan. New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling. Cellular & Molecular Biology Letters. 2024;29(1):100. 

Gal, Minju; Tuan, Ha Manh; Park, Ju-Hee; Park, Kang-Hyeon; Kim, Okhwa; Min, Byung-Sun; Lee, Jeong-Hyung. Irilin D suppresses RANKL-induced osteoclastogenesis and prevents inflammation-induced bone loss by disrupting the NF-κB and MAPK signaling pathways. European Journal Of Pharmacology. 2024;982:176956. 

Xue, Peng; Zhang, Weixin; Shen, Mengxi; Yang, Junhua; Chu, Jiachen; Wang, Shenyu; Wan, Mei; Zheng, Junying; Qiu, Zhaozhu; Cao, Xu. Proton-Activated Chloride Channel Increases Endplate Porosity and Pain in a Mouse Spinal Degeneration Model. The Journal Of Clinical Investigation. 2024; 39196784 

Döding, Annika; Güllich, Alexander; Koch, Simon; de Miroschedji, Kyra; Schulze-Späte, Ulrike. Human platelet-derived extracellular vesicle fractions modulate bone cell metabolism and biologize volume-stable β-TCP matrix in vitro. Bmc Medicine. 2025;23(1):569. 

Sasagawa, Karin; Domon, Hisanori; Hirayama, Satoru; Maekawa, Tomoki; Isono, Toshihito; Tabeta, Koichi; Terao, Yutaka. Secretory leucocyte protease inhibitor regulates bone metabolism and inflammation in experimental mouse periodontitis. Communications Biology. 2025;8(1):760. 

Jantarawong, Sompot; Khimmaktong, Wipapan; Swangphon, Piyawut; Lauterbach, Natda; Nanakorn, Natthaphon; Panichayupakaranant, Pharkphoom; Pengjam, Yutthana. Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Scientific Reports. 2025;15(1):21255. 

Leikina, Evgenia; Whitlock, Jarred M; Melikov, Kamran; Zhang, Wendy; Bachmann, Michael P; Chernomordik, Leonid V. FORMATION OF MULTINUCLEATED OSTEOCLASTS DEPENDS ON AN OXIDIZED SPECIES OF CELL SURFACE ASSOCIATED LA PROTEIN. Biorxiv : The Preprint Server For Biology. 2024; 38903088 

Feldman, George; Young, David; Freeman, Theresa; Israel, Lauren; Taub, Daniel; Diecidue, Robert. Geranylgeraniol (GGOH), incorporated into a bone cement pellet promotes osteoclast function and healing in a model of medication-related osteonecrosis of the jaw. Journal Of Oral Biology And Craniofacial Research. 2024;14(2):126-132. 

Chen, Zhihao; Yao, Hongyuan; Encarnacion, Alessandra Marie; Jeong, Jujin; Choi, Yunju; Park, Sangwook; Lee, Sunwoo; Lee, Taehoon. Novel Inhibitor of Keap1-Nrf2 Protein-Protein Interaction Attenuates Osteoclastogenesis In Vitro and Prevents OVX-Induced Bone Loss In Vivo. Antioxidants (Basel, Switzerland). 2024;13(7) 

de Castro, Luis F; Whitlock, Jarred M; Michel, Zachary; Pan, Kristen; Taylor, Jocelyn; Szymczuk, Vivian; Boyce, Brendan; Martin, Daniel; Kram, Vardit; Galisteo, Rebeca; Melikov, Kamran; Chernomordik, Leonid V; Collins, Michael T; Boyce, Alison M. RANKL inhibition reduces lesional cellularity and Gα(s) variant expression and enables osteogenic maturation in fibrous dysplasia. Bone Research. 2024;12(1):10. 

Liao, Runzhi; Dewey, Marley J; Rong, Jiayang; Johnson, Scott A; D'Angelo, William A; Hussey, George S; Badylak, Stephen F. Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis. Science Advances. 2024;10(42):eadn1852. 

Blümke, Alexander; Simon, Jessica; Leber, Elizabeth; Scatena, Marta; Giachelli, Cecilia M. Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells. Journal Of Visualized Experiments : Jove. 2024; 38587386(205) 

Dong, Junjie; Shu, Guizhao; Yang, Jin; Wang, Bing; Chen, Lingqiang; Gong, Zhiqiang; Zhang, Xiaofeng. Mechanistic study on the alleviation of postmenopausal osteoporosis by Lactobacillus acidophilus through butyrate-mediated inhibition of osteoclast activity. Scientific Reports. 2024;14(1):7042. 

Mohamad Hazir, Nur Shukriyah; Yahaya, Nor Hamdan Mohamad; Zawawi, Muhamad Syahrul Fitri; Damanhuri, Hanafi Ahmad; Mohamed, Norazlina; Alias, Ekram. Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis. International Journal Of Molecular Sciences. 2022;23(15) 

Ding, Lingli; Gao, Zhao; Wu, Siluo; Chen, Chen; Liu, Yamei; Wang, Min; Zhang, Yage; Li, Ling; Zou, Hong; Zhao, Guoping; Qin, Shengnan; Xu, Liangliang. Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress. Natural Products And Bioprospecting. 2023;13(1):49. 

Li, Junqin; Liu, Bin; Wu, Hao; Zhang, Shuaishuai; Liang, Zhuowen; Guo, Shuo; Jiang, Huijie; Song, Yue; Lei, Xing; Gao, Yi; Cheng, Pengzhen; Li, Donglin; Wang, Jimeng; Liu, Yang; Wang, Di; Zhan, Nazhi; Xu, Jing; Wang, Lin; Xiao, Guozhi; Yang, Liu; Pei, GuoXian. Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40). Bone Research. 2023;11(1):64. 

Tokuda, Kanako; Lu, Shiou-Ling; Zhang, Zidi; Kato, Yumiko; Chen, Siyu; Noda, Kazuya; Hirose, Katsutoshi; Usami, Yu; Uzawa, Narikazu; Murakami, Shinya; Toyosawa, Satoru; Fukuda, Mitsunori; Sun-Wada, Ge-Hong; Wada, Yoh; Noda, Takeshi. Rab32 and Rab38 maintain bone homeostasis by regulating intracellular traffic in osteoclasts. Cell Structure And Function. 2023;48(2):223-239. 

Whitlock, Jarred M; de Castro, Luis F; Collins, Michael T; Chernomordik, Leonid V; Boyce, Alison M. An inducible explant model of osteoclast-osteoprogenitor coordination in exacerbated osteoclastogenesis. Iscience. 2023;26(4):106470. 

Nakaue, Emiko; Teramachi, Jumpei; Tenshin, Hirofumi; Hiasa, Masahiro; Harada, Takeshi; Oda, Asuka; Inoue, Yusuke; Shimizu, So; Higa, Yoshiki; Sogabe, Kimiko; Oura, Masahiro; Hara, Tomoyo; Sumitani, Ryohei; Maruhashi, Tomoko; Yamagami, Hiroki; Endo, Itsuro; Tanaka, Eiji; Abe, Masahiro. Mechanisms of preferential bone formation in myeloma bone lesions by proteasome inhibitors. International Journal Of Hematology. 2023;118(1):88-98. 

Kim, Seong-Kyu; Choe, Jung-Yoon; Kim, Ji-Won; Park, Ki-Yeun. Histone Deacetylase 6 Inhibitor CKD-WID Suppressed Monosodium Urate-Induced Osteoclast Formation by Blocking Calcineurin-NFAT Pathway in RAW 264.7 Cells. Pharmaceuticals (Basel, Switzerland). 2023;16(3) 

Urabe, Fumihiko; Kosaka, Nobuyoshi; Yamamoto, Yusuke; Ito, Kagenori; Otsuka, Kurataka; Soekmadji, Carolina; Egawa, Shin; Kimura, Takahiro; Ochiya, Takahiro. Metastatic prostate cancer-derived extracellular vesicles facilitate osteoclastogenesis by transferring the CDCP1 protein. Journal Of Extracellular Vesicles. 2023;12(3):e12312. 

Rabjohns, Emily M; Rampersad, Rishi R; Ghosh, Arin; Hurst, Katlyn; Eudy, Amanda M; Brozowski, Jaime M; Lee, Hyun Ho; Ren, Yinshi; Mirando, Anthony; Gladman, Justin; Bowser, Jessica L; Berg, Kathryn; Wani, Sachin; Ralston, Stuart H; Hilton, Matthew J; Tarrant, Teresa K. Aged G Protein-Coupled Receptor Kinase 3 (Grk3)-Deficient Mice Exhibit Enhanced Osteoclastogenesis and Develop Bone Lesions Analogous to Human Paget's Disease of Bone. Cells. 2023;12(7) 

Akashi, Yoshihiro; Nagasaki, Atsuhiro; Okawa, Hiroko; Matsumoto, Takuya; Kondo, Takeru; Yatani, Hirofumi; Nishimura, Ichiro; Egusa, Hiroshi. Cyclic pressure-induced cytokines from gingival fibroblasts stimulate osteoclast activity: Clinical implications for alveolar bone loss in denture wearers. Journal Of Prosthodontic Research. 2023;67(1):77-86. 

Alam, Mohammad Ibtehaz; Mae, Megumi; Farhana, Fatima; Oohira, Masayuki; Yamashita, Yasunori; Ozaki, Yukio; Sakai, Eiko; Yoshimura, Atsutoshi. NLRP3 Inflammasome Negatively Regulates RANKL-Induced Osteoclastogenesis of Mouse Bone Marrow Macrophages but Positively Regulates It in the Presence of Lipopolysaccharides. International Journal Of Molecular Sciences. 2022;23(11) 

Mohamad Hazir, Nur Shukriyah; Yahaya, Nor Hamdan Mohamad; Zawawi, Muhamad Syahrul Fitri; Damanhuri, Hanafi Ahmad; Mohamed, Norazlina; Alias, Ekram. Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis. International Journal Of Molecular Sciences. 2022;23(15) 

Zang, Liqing; Kagotani, Kazuhiro; Nakayama, Hiroko; Bhagat, Jacky; Fujimoto, Yuki; Hayashi, Akihito; Sono, Ryoji; Katsuzaki, Hirotaka; Nishimura, Norihiro; Shimada, Yasuhito. 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales. Frontiers In Cell And Developmental Biology. 9:588093. 

Watahiki, Asami; Hoshikawa, Seira; Chiba, Mitsuki; Egusa, Hiroshi; Fukumoto, Satoshi; Inuzuka, Hiroyuki. Deficiency of Lipin2 Results in Enhanced NF-κB Signaling and Osteoclast Formation in RAW-D Murine Macrophages. International Journal Of Molecular Sciences. 2021;22(6) 

Steller, Daniel; Scheibert, Alexandra; Sturmheit, Tabea; Hakim, Samer G. Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes. Scientific Reports. 2020;10(1):16861. 

Chen, Zhihao; Cho, Eunjin; Ding, Mina; Seong, Jihyoun; Che, Xiangguo; Lee, Sunwoo; Park, Byung-Ju; Choi, Je-Yong; Lee, Tae-Hoon. N-[2-(4-benzoyl-1-piperazinyl)phenyl]-2-(4-chlorophenoxy) acetamide is a novel inhibitor of resorptive bone loss in mice. Journal Of Cellular And Molecular Medicine. 2021;25(3):1425-1438. 

Toung D. Development of a Cause-based Treatment for NBP-induced MRONJ. J Oral Maxillofac Surg. 2021;79(10):e51-e52. 

Steller, Daniel; Scheibert, Alexandra; Sturmheit, Tabea; Hakim, Samer G. Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes. Scientific Reports. 2020;10(1):16861. 

Kim, Hyun Young; Kim, Kyeong Seok; Kim, Myung Ji; Kim, Hyung-Shik; Lee, Kwang-Youl; Kang, Keon Wook. Auranofin Inhibits RANKL-Induced Osteoclastogenesis by Suppressing Inhibitors of κB Kinase and Inflammasome-Mediated Interleukin-1β Secretion. Oxidative Medicine And Cellular Longevity. 2019:3503912. 

Abdallah, Basem M; Ali, Enas M; Elsawy, Hany; Badr, Gehan M; Abdel-Moneim, Ashraf M; Alzahrani, Abdullah M. The Coumarin Derivative 5'-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways. Biomed Research International. 2019:9395146. 

Cho, Eunjin; Chen, Zhihao; Lee, Jinkyung; Lee, Sunwoo; Lee, Tae-Hoon. PSTP-3,5-Me Inhibits Osteoclast Differentiation and Bone Resorption. Molecules (Basel, Switzerland). 2019;24(18) 

Kim, Seonyoung; Kang, Seok-Seong; Choi, Soo-Im; Kim, Gun-Hee; Imm, Jee-Young. Ecklonia cava Extract Containing Dieckol Suppresses RANKL-Induced Osteoclastogenesis via MAP Kinase/NF-κB Pathway Inhibition and Heme Oxygenase-1 Induction. Journal Of Microbiology And Biotechnology. 2019;29(1):11-20. 

Chen, Zhihao; Cho, Eunjin; Lee, Jinkyung; Lee, Sunwoo; Lee, Tae-Hoon. Inhibitory Effects of N-[2-(4-acetyl-1-piperazinyl) phenyl]-2-(2-chlorophenoxy) acetamide on Osteoclast Differentiation In Vitro via the Downregulation of TRAF6. International Journal Of Molecular Sciences. 2019;20(20) 

Funaki, Yoshihiro; Hasegawa, Yasuyuki; Okazaki, Ryota; Yamasaki, Akira; Sueda, Yuriko; Yamamoto, Akihiro; Yanai, Masaaki; Fukushima, Takehito; Harada, Tomoya; Makino, Haruhiko; Shimizu, Eiji. Resolvin E1 Inhibits Osteoclastogenesis and Bone Resorption by Suppressing IL-17-induced RANKL Expression in Osteoblasts and RANKL-induced Osteoclast Differentiation. Yonago Acta Medica. 2018;61(1):8-18. 

Sapkota, Mahesh; Li, Liang; Kim, Se-Woong; Soh, Yunjo. Thymol inhibits RANKL-induced osteoclastogenesis in RAW264.7 and BMM cells and LPS-induced bone loss in mice. Food And Chemical Toxicology : An International Journal Published For The British Industrial Biological Research Association. 2018;120:418-429. 

Na, Sungsoo; TruongVo, ThucNhi; Jiang, Feifei; Joll, Jeffery E; Guo, Yunxia; Utreja, Achint; Chen, Jie. Dose analysis of photobiomodulation therapy on osteoblast, osteoclast, and osteocyte. Journal Of Biomedical Optics. 2018;23(7):1-8. 

Chu G. Punicalagin inhibits RANKL-induced osteoclastogenesis by suppressing NF- KB and MAPK signaling pathways. Int J Clin Exp Med . 2018;11(7):6571-6582. 

Lee, Sang-Hyun; Lee, Jung-Yun; Kwon, Young-In; Jang, Hae-Dong. Anti-Osteoclastic Activity of Artemisia capillaris Thunb. Extract Depends upon Attenuation of Osteoclast Differentiation and Bone Resorption-Associated Acidification Due to Chlorogenic Acid, Hyperoside, and Scoparone. International Journal Of Molecular Sciences. 2017;18(2) 

Lee, Da-Hye; Kim, Min Jung; Ahn, Jiyun; Lee, Sang Hee; Lee, Hyunjung; Kim, Jin Hee; Park, So-Hyun; Jang, Young-Jin; Ha, Tae-Youl; Jung, Chang Hwa. Nutrikinetics of Isoflavone Metabolites After Fermented Soybean Product (Cheonggukjang) Ingestion in Ovariectomized Mice. Molecular Nutrition & Food Research. 2017;61(12) 

Joung, Youn Hee; Darvin, Pramod; Kang, Dong Young; Sp, Nipin; Byun, Hyo Joo; Lee, Chi-Ho; Lee, Hak Kyo; Yang, Young Mok. Methylsulfonylmethane Inhibits RANKL-Induced Osteoclastogenesis in BMMs by Suppressing NF-κB and STAT3 Activities. Plos One. 11(7):e0159891. 

Cheng, Tsung-Lin; Lai, Chao-Han; Shieh, Shyh-Jou; Jou, Yin-Bo; Yeh, Jwu-Lai; Yang, Ai-Lun; Wang, Yan-Hsiung; Wang, Chau-Zen; Chen, Chung-Hwan; Shi, Guey-Yueh; Ho, Mei-Ling; Wu, Hua-Lin. Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss. Scientific Reports. 2016;6:28340. 

Li, Liang; Sapkota, Mahesh; Kim, Se-woong; Soh, Yunjo. Herbacetin inhibits RANKL-mediated osteoclastogenesis in vitro and prevents inflammatory bone loss in vivo. European Journal Of Pharmacology. 2016;777:17-25. 

Shiota J. Iguratimod, a Disease-Modifying Anti-Rheumatic Drug, Inhibits Osteoclastogenesis and Bone Resorption through Suppression of the Nuclear Factor of Activated T Cells Signaling Pathway. . Open Journal of Rheumatology and Autoimmune Diseases. 2016;6:106-119. 

Qiu, Zuo-Cheng; Dong, Xiao-Li; Dai, Yi; Xiao, Gao-Keng; Wang, Xin-Luan; Wong, Ka-Chun; Wong, Man-Sau; Yao, Xin-Sheng. Discovery of a New Class of Cathepsin K Inhibitors in Rhizoma Drynariae as Potential Candidates for the Treatment of Osteoporosis. International Journal Of Molecular Sciences. 2016;17(12) 

Ishikawa, Shintaro; Tamaki, Misako; Ogawa, Yui; Kaneki, Kiyomi; Zhang, Meng; Sunagawa, Masataka; Hisamitsu, Tadashi. Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells. Evidence-Based Complementary And Alternative Medicine : Ecam. 2016:7262054. 

Choe, Chung-Hyeon; Park, In Sun; Park, Jisang; Yu, Kang-Yeol; Jang, Hyonseok; Kim, Ju; Jang, Yong-Suk. Transmembrane protein 173 inhibits RANKL-induced osteoclast differentiation. Febs Letters. 2015;589(7):836-41. 

Sapkota, Mahesh; Li, Liang; Choi, Hyukjae; Gerwick, William H; Soh, Yunjo. Bromo-honaucin A inhibits osteoclastogenic differentiation in RAW 264.7 cells via Akt and ERK signaling pathways. European Journal Of Pharmacology. 2015;769:100-9. 

Nishida, Hiroko; Suzuki, Hiroshi; Madokoro, Hiroko; Hayashi, Mutsumi; Morimoto, Chikao; Sakamoto, Michiie; Yamada, Taketo. Blockade of CD26 signaling inhibits human osteoclast development. Journal Of Bone And Mineral Research : The Official Journal Of The American Society For Bone And Mineral Research. 2014;29(11):2439-55. 

Kang, J H; Ko, H M; Moon, J S; Yoo, H I; Jung, J Y; Kim, M S; Koh, J T; Kim, W J; Kim, S H. Osteoprotegerin expressed by osteoclasts: an autoregulator of osteoclastogenesis. Journal Of Dental Research. 2014;93(11):1116-23. 

Tanaka, Hideki; Tanabe, Natsuko; Kawato, Takayuki; Nakai, Kumiko; Kariya, Taro; Matsumoto, Sakurako; Zhao, Ning; Motohashi, Masafumi; Maeno, Masao. Nicotine affects bone resorption and suppresses the expression of cathepsin K, MMP-9 and vacuolar-type H(+)-ATPase d2 and actin organization in osteoclasts. Plos One. 8(3):e59402. 

Kim, Jung-Lye; Kang, Sang-Wook; Kang, Min-Kyung; Gong, Ju-Hyun; Lee, Eun-Sook; Han, Seoung Jun; Kang, Young-Hee. Osteoblastogenesis and osteoprotection enhanced by flavonolignan silibinin in osteoblasts and osteoclasts. Journal Of Cellular Biochemistry. 2012;113(1):247-59. 

Leikina, Evgenia; Whitlock, Jarred M; Melikov, Kamran; Zhang, Wendy; Bachmann, Michael P; Chernomordik, Leonid. Formation of multinucleated osteoclasts depends on an oxidized species of cell surface-associated La protein. Elife. 2024;13