Blockmaster PA1080

MBL Life Sciences

Catalog No.: JSR-J-PA1080

Product Description

PREVENT NON-SPECIFIC ADSORPTION OF PROTEINS AND CELLS
Blockmaster™ PA1080 is a fully synthetic, water soluble polymer blocking reagent consisting of both hydrophilic and hydrophobic units. The optimized combination of hydrophilic and hydrophobic units realizes excellent protein/cell blocking properties on a wide variety of substrate materials.

Key Properties

• Coating by physical adsorption to different substrate materials — PSt, PP, COP, Glass, PDMS, PVDF, Nitro cellulose, etc.
• Simple coating process
• Prevention of non-specific adsorption of proteins/cells
• Various applications: Microfluidics, 3D cell culture, ELISA Plate, etc.
Hydrophillic unit
Hydrophobic unit
Polymer Design
Protocol

Example Protocol for a Polystyrene 96-Well Plate

1. Add 200 μL of Blockmaster™ PA1080* per well
2. Incubate for 30 min at room temperature and remove
3. Wash the wells x 3 with 350 μL water (proteins) or PBS (cells)
4. Add 100 μL protein or cell solution into the wells
 Blockmaster™ PA1080 coating prevents protein/cell adsorption to the wells
* Recommended concentration is 0.1%-1% but this has to be optimized for each application

 

Documentation Requirements for Order Placement

Research Use Only Agreement (RUO)
The manufacturer of this product requires a signed Research Use Only Agreement to be submitted at the time of order placement. Access the agreement in the Documents section above. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.