PREVENT NON-SPECIFIC ADSORPTION OF PROTEINS AND CELLS Blockmaster™ PA1080 is a fully synthetic, water soluble polymer blocking reagent consisting of both hydrophilic and hydrophobic units. The optimized combination of hydrophilic and hydrophobic units realizes excellent protein/cell blocking properties on a wide variety of substrate materials. Key Properties • Coating by physical adsorption to different substrate materials — PSt, PP, COP, Glass, PDMS, PVDF, Nitro cellulose, etc. • Simple coating process • Prevention of non-specific adsorption of proteins/cells • Various applications: Microfluidics, 3D cell culture, ELISA Plate, etc. Hydrophillic unit Hydrophobic unit Polymer Design Protocol Example Protocol for a Polystyrene 96-Well Plate 1. Add 200 μL of Blockmaster™ PA1080* per well 2. Incubate for 30 min at room temperature and remove 3. Wash the wells x 3 with 350 μL water (proteins) or PBS (cells) 4. Add 100 μL protein or cell solution into the wells Blockmaster™ PA1080 coating prevents protein/cell adsorption to the wells * Recommended concentration is 0.1%-1% but this has to be optimized for each application
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