Blockmaster CE510

MBL Life Sciences

Catalog No.: JSR-J-CE510RAN

FULLY CHEMICAL SYNTHESIZED BLOCKING REAGENT
Blockmaster™ CE510 and CE210 are fully synthetic, water soluble blocking reagents consisting of a hydrophilic PEG tail and multiple amine-end groups. While the PEG tail reduces the non-specific binding, the amine end-group allows for a covalent conjugation with amine reactive surfaces.
Key Properties
• A unique composition consisting of a hydrophilic PEG-tail and 
multiple amine end-groups
• High durability by covalent coupling 
• Low non-specific binding of proteins/cells
• Signal enhancement thanks to improved antibody orientation 
• Improvement of beads dispersion

Example Protocol For Covalent Coupling
1. Magnetic beads 10 mg (1%, 1000 μL or 10% 100 μL) remove supernatant with magnet 
2. Mix Magnetic beads and Binding Buffer* 500 μL to pre-wash (Suspend the beads by vortexing. Then, remove supernatant with magnet) 
3. Mix Magnetic beads and Binding Buffer* 900 μL at 25˚C 
4. Add 1% EDC** 100 μL, mixing at 25˚C for 30 min 
5. Add antibody 1 mg/mL 100 μL, mixing at 25˚C for 1–3 hours 
6. Add Blocking solution 500 μL (2 wt% Blockmaster™ CE510) mixing at 25˚C for 1–3 hours 
7. Remove the supernatant by magnet 
8. Add Washing Buffer 500 μL, washing 4 times 
9. Add desired Buffer, suspending the beads by vortexing 
10. Store at 2–8˚C
*MagnosphereTM carboxyl beads

References
1. Thangavel Lakshmipriya, Makoto Fujimaki, Subash C.B. Gopinath, Koichi Awazu, Yukichi Horiguchi and Yukio Nagasaki, High-performance waveguide-mode biosensor for detection of Factor IX uses PEG-based blocking agents to suppress 
non-specific binding and improve sensitivity, Analyst, in press (DOI: 10.1039/C3AN00298E).
2. Xiaofei Yuan, Dolça Fabregat, Keitaro Yoshimoto and Yukio Nagasaki, High PEGylation Efficiency of Pentaethylenehexamine-end Poly(ethyleneglycol) (mPEG-N6) for Active-ester Surface, Colloid and Surface B: Biointerface, 92, 25-29 (2012) (DOI: 10.1016/j.colsurfb.2011.11.013).
3. Xiaofei Yuan, Dolça Fabregat, Keitaro Yoshimoto and Yukio Nagasaki: Development of a high-performance immunolatex based on “soft landing” antibody immobilization mechanism, Colloid and Surface B: Biointreface, Vol. 
9945-52(2012)(10.1016/j.colsurfb.2011.09.040).
4. Masaki Kubota, Keitaro Yoshimoto, Yuan Xiaofei, Yukio Nagasaki: Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed poly(ethylene glycol) tethered-chain layer, Polymer 
Journal, 43, 493-496 (2011).
5. Xiaofei Yuan, Dolça Fabregat, Keitaro Yoshimoto, Yukio Nagasaki, Design of Highly Functional Antiferritin-Immunolatex by Hybridization of Antiferritin/MixedPEG Polymers onto Polystyrene Submicroparticles, Biomaterials Chapter 13, pp 
243–258 ACS Symposium Series, Vol. 1054 (2010).
6. Yuan Xiaofei, Fabregat Dolça, Yoshimoto Keitaro, Nagasaki Yukio: Efficient Inhibition of Interfacial Nonspecific Interaction to Create Practically Utilizable High Ferritin-Response Immunolatex, Analytical Chemistry, 81 10097-10105 (2009).
7. Yuan Xiaofei, Yoshimoto Keitaro, Nagasaki Yukio: High-performance Immunolatex Possessing A Mixed-PEG/Antibody Co-immobilized Surface: High Sensitive Ferritin Immunodiagnostics. Analytical Chemistry: 81(4),1549-1556(2009).
8. Furusho Hitoshi, Kitano Katsuhisa, Hamaguchi Satoshi, Nagasaki Yukio: Preparation of Stable Water-Dispersible PEGylated Gold Nanoparticles Assisted by Nonequilibrium Atmospheric-Pressure Plasma Jets. Chemistry of Materials: 
21(15).3526-3535 (2009).
9. Nagasaki Yukio, Kobayashi Hiroshi, Katsuyama Yoshinori, Jomura Tomoko, Sakura Takeshi: Enhanced immunoresponse of antibody/mixed-PEG co-immobilized surface construction of high-performance immunomagnetic ELISA system. 
Journal of Colloid and Interface Science 309: 524-530 (2007).

Documentation Requirements for Order Placement

Research Use Only Agreement (RUO)
The manufacturer of this product requires a signed Research Use Only Agreement to be submitted at the time of order placement. Access the agreement in the Documents section below. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.