Anti TOP2A mAb (Clone 5B10)

CUSABIO

Catalog No.:
CSB-RA696677A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate<br /> All lanes: TOP2A antibody at 1:1500<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 175, 178, 179, 183 kDa<br /> Observed band size: 175 kDa<br />
IHC image of CSB-RA696677A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

Product Specifications
Application WB, IHC, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 5B10)

Documents & Links for Anti TOP2A mAb (Clone 5B10)
Datasheet Anti TOP2A mAb (Clone 5B10) Datasheet

Documents & Links for Anti TOP2A mAb (Clone 5B10)
Datasheet Anti TOP2A mAb (Clone 5B10) Datasheet
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