Anti SPP1 mAb (Clone 10A1)

CUSABIO

Catalog No.:
CSB-RA261140A0HU-50
Western Blot<br /> Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate<br /> All lanes: Osteopontin Antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 36, 34, 33, 34, 34 kDa<br /> Observed band size: 36 kDa<br />
IHC image of CSB-RA261140A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 10A1)

Documents & Links for Anti SPP1 mAb (Clone 10A1)
Datasheet Anti SPP1 mAb (Clone 10A1) Datasheet

Documents & Links for Anti SPP1 mAb (Clone 10A1)
Datasheet Anti SPP1 mAb (Clone 10A1) Datasheet
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