Anti SAE1 mAb (Clone 9D8)

CUSABIO

Catalog No.:
CSB-RA987582A0HU-50
Western Blot<br /> Positive WB detected in: K562 whole cell lysate<br /> All lanes: SAE1 Antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 39, 30, 34 kDa<br /> Observed band size: 39 kDa<br />
Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Product Specifications
Application WB, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 9D8)

Documents & Links for Anti SAE1 mAb (Clone 9D8)
Datasheet Anti SAE1 mAb (Clone 9D8) Datasheet

Documents & Links for Anti SAE1 mAb (Clone 9D8)
Datasheet Anti SAE1 mAb (Clone 9D8) Datasheet
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