Anti Phospho-STAT1 (S727) mAb (Clone 2H10)

CUSABIO

Catalog No.:
CSB-RA022810A727phHU-50
Western Blot<br /> Positive WB detected in HepG2 whole cell lysate(treated with Calyculin A or not)<br /> All lanes Phospho-STAT1 antibody at 1.065μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 87 KDa<br /> Observed band size: 87 KDa<br />
IHC image of CSB-RA022810A727phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells(treated with 100mM Calyculin A for 30min) with CSB-RA022810A727phHU at 1:66,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2H10)

Documents & Links for Anti Phospho-STAT1 (S727) mAb (Clone 2H10)
Datasheet Anti Phospho-STAT1 (S727) mAb (Clone 2H10) Datasheet

Documents & Links for Anti Phospho-STAT1 (S727) mAb (Clone 2H10)
Datasheet Anti Phospho-STAT1 (S727) mAb (Clone 2H10) Datasheet
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