Anti Phospho-PTPN11 (Y542) mAb (Clone 2E2)

CUSABIO

Catalog No.:
CSB-RA019025A542phHU-50
Western Blot<br /> Positive WB detected in Hela whole cell lysate锛?93 whole cell lysate锛孉549 whole cell lysate(treated with Pervanadate or not)<br /> All lanes Phospho-PTPN11 antibody at 0.65μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 68 KDa<br /> Observed band size: 68 KDa<br />
Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with Pervanadate<br /> Lane 1: Rabbit control IgG(1μg)instead of CSB-RA019025A542phHU in Hela whole cell lysate treated with Pervanadate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA019025A542phHU(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg)<br /> Lane 3: Hela whole cell lysate treated with Pervanadate(20μg)<br />
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Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).

Product Specifications
Application WB, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 200)

Documents & Links for Anti Phospho-PTPN11 (Y542) mAb (Clone 2E2)
Datasheet Anti Phospho-PTPN11 (Y542) mAb (Clone 2E2) Datasheet

Documents & Links for Anti Phospho-PTPN11 (Y542) mAb (Clone 2E2)
Datasheet Anti Phospho-PTPN11 (Y542) mAb (Clone 2E2) Datasheet
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