Anti Phospho-NFE2L2 (S40) mAb (Clone 2E6)

CUSABIO

Catalog No.:
CSB-RA614961A40phHU-50
Western Blot<br /> Positive WB detected in HepG2 whole cell lysate,293 whole cell lysate(treated with Calyculin A or EGF)<br /> All lanes Phospho-NFE2L2 antibody at 0.8μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 90 KDa<br /> Observed band size: 90 KDa<br />
IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-RA614961A40phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2000000)

Documents & Links for Anti Phospho-NFE2L2 (S40) mAb (Clone 2E6)
Datasheet Anti Phospho-NFE2L2 (S40) mAb (Clone 2E6) Datasheet

Documents & Links for Anti Phospho-NFE2L2 (S40) mAb (Clone 2E6)
Datasheet Anti Phospho-NFE2L2 (S40) mAb (Clone 2E6) Datasheet
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