Anti Phospho-JUN (S63) mAb (Clone 4A11)

CUSABIO

Catalog No.:
CSB-RA011972A63phHU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF)<br /> All lanes: Phospho-JUN antibody at 0.95&mu;g/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 48 KDa<br /> Observed band size: 48 KDa
IHC image of CSB-RA011972A63phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4&deg;C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-RA011972A63phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4&deg;C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 4A11)

Documents & Links for Anti Phospho-JUN (S63) mAb (Clone 4A11)
Datasheet Anti Phospho-JUN (S63) mAb (Clone 4A11) Datasheet

Documents & Links for Anti Phospho-JUN (S63) mAb (Clone 4A11)
Datasheet Anti Phospho-JUN (S63) mAb (Clone 4A11) Datasheet
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