Anti Phospho-Histone H2AX (S139) mAb (Clone 1F10)

CUSABIO

Catalog No.:
CSB-RA010097A139phHU-50
Western Blot<br /> Positive WB detected in:293 whole cell lysate<br /> All lanes:Phospho-Histone H2AX (S139) antibody at 0.23μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 15 KDa<br /> Observed band size: 15 KDa
IHC image of CSB-RA010097A139phHU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4&deg;C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Product Specifications
Application WB, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 1F10)

Documents & Links for Anti Phospho-Histone H2AX (S139) mAb (Clone 1F10)
Datasheet Anti Phospho-Histone H2AX (S139) mAb (Clone 1F10) Datasheet

Documents & Links for Anti Phospho-Histone H2AX (S139) mAb (Clone 1F10)
Datasheet Anti Phospho-Histone H2AX (S139) mAb (Clone 1F10) Datasheet
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