Anti Phospho-Histone H1.4 (T17) mAb (Clone 3E1)

CUSABIO

Catalog No.:
CSB-RA010380A17phHU-50
IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-RA010380A17phHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Product Specifications
Application IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 30)

Documents & Links for Anti Phospho-Histone H1.4 (T17) mAb (Clone 3E1)
Datasheet Anti Phospho-Histone H1.4 (T17) mAb (Clone 3E1) Datasheet

Documents & Links for Anti Phospho-Histone H1.4 (T17) mAb (Clone 3E1)
Datasheet Anti Phospho-Histone H1.4 (T17) mAb (Clone 3E1) Datasheet
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